Overview

This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Full Product Details

Author:   A. Kristina Downing
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2004
Volume:   278
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.874kg
ISBN:  

9781588292469

ISBN 10:   1588292460
Pages:   487
Publication Date:   17 August 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Screening and Optimizing Protein Production in E. coli Lorraine Hewitt and James M. McDonnell Isotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastoris Andrew R. Pickford and Joanne M. O'Leary Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins Stephen Matthews Segmental Isotopic Labeling for Structural Biological Applications of NMR David Cowburn, Alexander Shekhtman, Rong Xu, Jennifer J. Ottesen, and Tom W. Muir TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins Guang Zhu, Youlin Xia, Donghai Lin, and Xiaolian Gao Media for Studies of Partially Aligned States Kieran Fleming and Stephen Matthews Residual Dipolar Couplings in Protein Structure Determination Eva de Alba and Nico Tjandra Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules Christian Griesinger, Wolfgang Peti, Jens Meiler, and Raphael Brüschweiler Characterizing Domain Interfaces by NMR Luke M. Rooney, Sachchidanand, and Jörn M. Werner Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations Jennifer Blake Hall, Olivier Walker, and David Fushman TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding Guang Zhu, Youlin Xia, Donghai Lin, and Xiaolian Gao Measurement of Intermediate Exchange Phenomena James G. Kempf and J. Patrick Loria NMR Studies of Partially Folded Molten-Globule States Christina Redfield Structure Determination of Protein Complexes by NMR Daniel Nietlispach, Helen R. Mott, Katherine M. Stott, Peter R. Nielsen, Abarna Thiru, and Ernest D. Laue NMR Studies of Protein-Nucleic Acid Interactions Gabriele Varani, Yu Chen, andThomas C. Leeper Using NMRView to Visualize and Analyze the NMR Spectra of Macromolecules Bruce A. Johnson Automated NMR Structure Calculation With CYANA Peter Güntert NOE Assignment With ARIA 2.0: The Nuts and Bolts Michael Habeck, Wolfgang Rieping, Jens P. Linge, and Michael Nilges Membrane Protein Structure Determination Using Solid-State NMR Anthony Watts, Suzana K. Straus, Stephan L. Grage, Miya Kamihira, Yuen Han Lam, and Xin Zhao Index

Reviews

From reviews of the first edition... ...includes many good practical details which are so often omitted and which workers new to the field have to rediscover... -Analyst ...an excellent text... a very useful compilation...The limitations and power of the approaches are well presented and should help in experimental design very considerably. -Journal of Medicinal Chemistry ...a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. -Trends in Biotechnology ...an excellent introduction...Readers will be left with a good understanding of the application of NMR to the study of proteins. -Quarterly Review of Biology

From reviews of the first edition... ...includes many good practical details which are so often omitted and which workers new to the field have to rediscover... -Analyst ...an excellent text... a very useful compilation...The limitations and power of the approaches are well presented and should help in experimental design very considerably. -Journal of Medicinal Chemistry ...a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. -Trends in Biotechnology ...an excellent introduction...Readers will be left with a good understanding of the application of NMR to the study of proteins. -Quarterly Review of Biology

From reviews of the first edition includes many good practical details which are so often omitted and which workers new to the field have to rediscover - Analyst an excellent text a very useful compilation The limitations and power of the approaches are well presented and should help in experimental design very considerably. - Journal of Medicinal Chemistry a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. - Trends in Biotechnology an excellent introduction Readers will be left with a good understanding of the application of NMR to the study of proteins. - Quarterly Review of Biology

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