Overview

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest.  Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise. Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Ranajeet Ghose
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1688
Weight:   1.403kg
ISBN:  

9781493973859

ISBN 10:   1493973851
Pages:   446
Publication Date:   19 November 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology.- Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-state NMR of Microcrystalline and Membrane-Bound Proteins.- Afterglow Solid-State NMR Spectroscopy.- Filamentous Bacteriophage Viruses: Preparation, Magic-angle Spinning Solid-state NMR Experiments and Structure Determination.- Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes.- Rapid Prediction of Multi-dimensional NMR Data Sets using FANDAS.- Strategies for Efficient Sample Preparation for Dynamic Nuclear Polar­ization Solid State NMR of Biological Macromolecules.- In-vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules.- Determination of Protein ps-ns Motions by High-Resolution Relaxometry.- Characterizing Protein Dynamics with NMR R1r Relaxation Experiments.- CPMG Experiments for Protein Minor Conformer Structure Determination.- Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR.- From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis.- Protein Structure Elucidation from NMR Data with the Program Xplor-NIH.- Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR.- Covariance NMR Processing and Analysis for Protein Assignment.- Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System.- Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist’s Approach to Allostery.- High-efficiency Expression of Yeast-derived G Protein-coupled Receptors and 19F Labeling for Dynamical Studies.- Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-cell NMR Spectroscopy. 

Reviews

The book covers current applications of solid-state and solution nuclear magnetic resonance spectroscopy to study protein structure, dynamics. and interactions. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols with tips to tackle troubleshooting. The book has several high quality illustrations in color or black and white. ... The intended audience appears to be students, specialists, and those who are interested in NMR spectroscopy. (Omer Iqbal, Doody's Book Reviews, April, 2018)

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