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Overview"There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable ""tricks of the trade"" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user." Full Product DetailsAuthor: Viki Allan (School of Biological Sciences, University of Manchester)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 218 Dimensions: Width: 15.50cm , Height: 1.40cm , Length: 23.40cm Weight: 0.440kg ISBN: 9780199637409ISBN 10: 0199637407 Pages: 256 Publication Date: 18 November 1999 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsPreface 1: Allan: Basic Immunofluorescence 2: North and Small: Immunofluorescent Labelling of Sections 3: Ersfeld and Stone: 4: Amos: Instruments for Fluorescence Imaging 5: Pepperkok and Shima: Fluorescence Microscopy of Living Vertebrate Cells 6: Davis: Visualising Fluorescence in Drosophila - optimal detection in thick specimens 7: Hawes, Boevink and Moore: Green Fluorescent Protein in Plants 8: Hagan and Ayscough: Fluorescence Microscopy in Yeast IndexReviews... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text * Trends Cell Biology * ... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text Trends Cell Biology Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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