Protein Folding: Methods and Protocols

Author:   Victor Muñoz
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2376
ISBN:  

9781071617151


Pages:   419
Publication Date:   30 November 2021
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Protein Folding: Methods and Protocols


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Author:   Victor Muñoz
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2376
Weight:   1.005kg
ISBN:  

9781071617151


ISBN 10:   107161715
Pages:   419
Publication Date:   30 November 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Mutational Analysis of Protein Folding Transition States: Phi-values.- Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi Analysis.- Site-Specific Interrogation of Protein Structure and Stability.- Purification and Handling of the Chaperonin GroEL.- ­Folding free energy surfaces from Differential Scanning Calorimetry.- Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump Methods7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical Oxidation.- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding.- Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes.- Nmr Analysis Of Protein Folding Interaction Networks.- NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-States.-  Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding.- Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and Interactions.- Theory and Analysis of Single-Molecule FRET experiments.- Mechanochemical Evolution Of Disulfide Bonds In Proteins.- Coarse-Grained Simulations of Protein Folding: Bridging Theory and Experiments.- Analysis of Molecular Dynamics Simulations of Protein Folding.- Atomistic Simulations of Thermal Unfolding.- Molecular Simulations of Intrinsically Disordered Proteins and their Binding Mechanisms.- Prediction of Folding and Unfolding Rates of Proteins with Simple Models.- Predicting and Simulating Mutational Effects on Protein Folding Kinetics.- Localization of Energetic Frustration in Proteins.- Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES  Force Field.

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