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OverviewThis volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. Full Product DetailsAuthor: Victor MuñozPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2022 Volume: 2376 Weight: 0.817kg ISBN: 9781071617182ISBN 10: 1071617184 Pages: 419 Publication Date: 30 November 2022 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Mutational Analysis of Protein Folding Transition States: Phi-valuesLuis Alberto Campos 2. Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi Analysis Michael Baxa, Tobin R. Sosnick 3. Site-Specific Interrogation of Protein Structure and Stability Debopreeti Mukherjee, Ismail A. Ahmed, and Feng Gai 4. Purification and Handling of the Chaperonin GroEL Xiang Ye 5. Folding free energy surfaces from Differential Scanning Calorimetry Jose M. Sanchez-Ruiz and Beatriz Ibarra-Molero 6. Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump Methods Michele Cerminara 7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical Oxidation David Witalka and Lisa J. Lapidus 8. Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding Pooja Malhotra and Jayant B. Udgaonkar 9. Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes Ginka S. Kubelka and Jan Kubelka 10. Nmr Analysis Of Protein Folding Interaction Networks Eva de Alba 11. NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-States V. N. Sivanandam, Nicola D’Amelio, and Victor Muñoz 12. Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding Pooja Malhotra and Jayant B. Udgaonkar 13. Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and Interactions Zifan Wang, Nivin Mothi, and Victor Muñoz 14. Theory and Analysis of Single-Molecule FRET experiments Irina V. Gopich and Hoi Sung Chung 15. Mechanochemical Evolution Of Disulfide Bonds In Proteins Jörg Schönfelder, Alvaro Alonso-Caballero, and Raul Perez-Jimenez 16. Coarse-Grained Simulations of Protein Folding: Bridging Theory and Experiments Vinícius G. Contessoto, Vinícius M. Oliveira, and Vitor B. P. Leite 17. Analysis of Molecular Dynamics Simulations of Protein Folding Robert B. Best 18. Atomistic Simulations of Thermal Unfolding Angel E Garcia 19. Molecular Simulations of Intrinsically Disordered Proteins and their Binding Mechanisms Xiakun Chu, Suhani Nagpal, and Victor Muñoz 20. Prediction of Folding and Unfolding Rates of Proteins with Simple Models David De Sancho and Victor Munoz 21. Predicting and Simulating Mutational Effects on Protein Folding Kinetics Athi N. Naganathan 22. Localization of Energetic Frustration in Proteins A. Brenda Guzovsky, Nicholas P. Schafer, Peter G. Wolynes, and Diego U. Ferreiro 23. Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES Force Field Adam K. Sieradzan, Cezary Czaplewski, P aweł Krupa, Magdalena A. Mozolewska, Agnieszka Karczyńska, Agnieszka Lipska, Emilia A. Lubecka, Ewa Gołaś, Tomasz Wirecki, Mariusz Makowski, Stanisław Ołdziej, and Adam LiwoReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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