Overview

This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry. 

Full Product Details

Author:   Nikolaos E. Labrou
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2178
Weight:   0.994kg
ISBN:  

9781071607770

ISBN 10:   1071607774
Pages:   511
Publication Date:   01 November 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Protein Purification Technologies.- High Throughput Process Development: I. Process Chromatography.- 3. High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers.- Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification.- Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening.- ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification.- Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing.- Sample Displacement Batch Chromatography of Proteins.- Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3.- Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins.- Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+.- Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin.- Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry.- Protein Structure Analysis and Validation with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification.- Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays.

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