Overview

This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Cutting-edge and thorough, Protein Arginylation: Methods and Protocols, Second Edition  aims to be of interest to novice researchers involved in posttranslational arginylation research and researchers working on a broad range of posttranslational modifications.

Full Product Details

Author:   Anna S. Kashina
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2620
Weight:   0.787kg
ISBN:  

9781071629413

ISBN 10:   1071629417
Pages:   297
Publication Date:   04 April 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Protein arginylation: milestones of discovery Anna Kashina 2. Recollection of how we came across the protein modification with amino acids by aminoacyl tRNA-protein transferase Hideko Kaji and Akira Kaji   3. Arginyltransferase: a personal and historical perspective Richard L. Soffer   4. Arginylation in a Partially Purified Fraction of 150,000xg Supernatants of Axoplasm and Injured Vertebrate Nerves Nicholas A. Ingoglia   5. Preparation of ATE1 enzyme from native mammalian tissues Anna Kashina   6. Correlated measurement of endogenous ATE1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging Hideko Kaji and Akira Kaji   7. Assaying the Post-translational Arginylation of Proteins in Cultured Cells Mauricio R. Galiano and Marta E. Hallak   8. Assaying ATE1 activity in yeast by β-gal degradation Anna Kashina.   9. Assaying arginylation activity in cell lysates using a fluorescent reporter Akhilesh Kumar and Fangliang Zhang.   10. Assaying intracellular arginylation activity using a fluorescent reporter. Brittany MacTaggart and Anna Kashina   11. Bacterial expression and purification of recombinant arginyltransferase (ATE1) and Arg-tRNA synthetase (RRS) for arginylation assays  Junling Wang and Anna Kashina   12. Preparation of tRNAArg for arginylation assay by in vitro transcription by Irem Avcilar-Kucukgoze and Anna Kashina   13. Preparation of an enriched tRNAArg fraction for arginylation by expression in E. coli Irem Avcilar-Kucukgoze, Howard Gamper, Jr., Ya-Ming Hou, and Anna Kashina   14. Enzymatic aminoacylation of tRNAArg using recombinant Arg-tRNA synthetase Irem Avcilar-Kucukgoze and Anna Kashina   15. Assaying ATE1 activity in vitro  Junling Wang and Anna Kashina.   16. High throughput arginylation assay in microplate format Sougata Saha, Junling Wang, and Anna Kashina   17. Assaying for arginyltransferase activity and specificity by peptide arrays Junling Wang and Anna Kashina.   18. Assay of arginyltransferase activity by a fluorescent HPLC method  Koichi Takao   19. Identification of arginylated proteins by mass spectrometry Anna Kashina and John R. Yates, III   20. Analysis of arginylated peptides by subtractive Edman degradation. Anna Kashina and John R. Yates, III 21. Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, and E. James Petersson.   22. Synthesis of peptides and proteins with site-specific glutamate arginylation Marie Shimogawa, Yun Huang, Buyan Pan, and E. James Petersson.   23. Reconstitution of the arginyltransferase (ATE1) iron-sulfur cluster Verna Van and Aaron T. Smith.   24. N-terminal arginylation pulldown analysis using the R-catcher tool Taewook Seo, Goeun Han, and Hyunjoo Cha-Molstad   25. Identification of protein arginylation by encapsulated N-terminal peptide enrichment method  Shinyeong Ju and Cheolju Lee.   26. Monitoring the Activation of Selective Autophagy via N-terminal Arginylation. Chang Hoon Ji, Su Bin Kim, Min Ju Lee, and Yong Tae Kwon   27. Analyzing the Interaction of Arginylated Proteins and Nt-Arg-Mimicking Chemical Compounds to N-Recognins Chang Hoon Ji, Ah Jung Heo, and Yong Tae Kwon 28 Synthesis of Stably Charged Arg-tRNAArg for Structural Analysis Yuka Yamaki, Howard Gamper, and Ya-Ming Hou   29. A High-Throughput Colorimetric Microplate Assay for Determination of Plasma Arginase Activity Natalie J Smith, Mahnaz Maddahfar, Bavani Gunasegaran  Helen M McGuire, and Barbara Fazekas de St Groth.   30 Development of new tools for the studies of protein arginylation Anna Kashina

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