Protein Aggregation: Methods and Protocols

Author:   Andrzej Stanisław Cieplak
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2551
ISBN:  

9781071625965


Pages:   681
Publication Date:   31 October 2022
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Protein Aggregation: Methods and Protocols


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Author:   Andrzej Stanisław Cieplak
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2551
Weight:   1.496kg
ISBN:  

9781071625965


ISBN 10:   1071625969
Pages:   681
Publication Date:   31 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Early aggregation of Amyloid-β (1-42) studied by Fluorescence Correlation Spectroscopy Mercedes Novo, Cibrán Pérez-González, Sonia Freire, and Wajih Al-Soufi   2. Preparation and investigation of crucial oligomers in the early stages of Aβ40 and Aβ42                 aggregation Bertrand Morel and Francisco Conejero-Lara   3. Preparation and fractionation of heterogeneous Aβ42 oligomers with different aggregation properties Erica W. Chen and Zhefeng Guo   4. An efficient method of expression and purification of amyloid beta (Aβ1-42) peptide from E.coli Dhiman Ghosh, Marielle Aulikki Wälti, and Roland Riek   5. Solid-state NMR structure of amyloid-β fibrils Beat H. Meier and Anja Böckmann   6. Time-resolved in situ AFM measurement of growth rates of Aβ40 fibrils Peter G. Vekilov and Peter G. Wolynes   7. Monitoring kinetics of pH-dependent aggregation and disaggregation of the Pmel17 repeat domain Dexter N. Dean and Jennifer C. Lee   8.Analysis of Tau:nucleoporin interactions by Surface Plasmon Resonance Spectroscopy Lisa Diez, Larisa E. Kapinos, Roderick Y. H. Lim, and Susanne Wegmann   9. Microfluidic chamber technology to study missorting and spreading of Tau protein in Alzheimer disease Senthilvelrajan Kaniyappan, Varun Balaji, Yipeng Wang, and Eckhard Mandelkow   10. Using FRET-based biosensor cells to study the seeding activity of tau and α-synuclein Katherine N. Maina, Caroline Smet-Nocca, and Gal Bitan   11. Functional applications of stable tau oligomers in cell biology and electrophysiology studies Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari   12. An additive-free model for tau self-assembly Youssra K Al-Hilaly, Karen E Marshall, Liisa Lutter, Luca Biasetti, Kurtis Mengham, Charles R Harrington, Wei-Feng Xue, Claude M Wischik, and Louise C Serpell   13. Cross-linking mass spectrometry analysis of metastable compact structures in intrinsically disordered proteins Dailu Chen and Lukasz A. Joachimiak   14. A validated method to prepare stable tau oligomers Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari   15. Light microscopy and dynamic light scattering to study liquid-liquid phase separation of Tau proteins in vitro Janine Hochmair, Christian Exner, Christian Betzel, Eckhard Mandelkow, and Susanne Wegmann   16. Study of tau liquid-liquid phase separation in vitro Solomiia Boyko and Witold K. Surewicz   17. Liquid-Liquid Phase Separation to study the association of proteins in solution Irving Vega and Andrew Umstead   18. Mapping phase diagram of tau-RNA LLPS under live cell coculturing conditions Yanxian Lin, Yann Fichou, Jennifer N. Rauch, Xuemei Zhang, Kenneth S. Kosik, and Songi Han   19. The role of buffers in wild-type HEWL amyloid fibril formation mechanism - a methodological approach Sandi Brudar and Barbara Hribar-Lee   20. Reproducible formation of insulin superstructures: amyloid-like fibrils, spherulites and particulates Camilla Thorlaksen, Martin Busch Neergaard, Minna Groenning, and Vito Foderà   21. CD and solid-state NMR studies of low-order oligomers of transthyretin Anvesh K. R. Dasari and Kwang Hun Lim   22. Identifying biological and biophysical features of different maturation states of α-synuclein amyloid fibrils Thomas Skamris, Bente Vestergaard, Kenneth L. Madsen, Annette E. Langkilde, and Vito Foderà   23. Preparation of α-synuclein fibril, ribbon and fibril-91 amyloid polymorphs for structural studies Luc Bousset, Ania Alik, Ana Arteni, Anja Böckmann, Beat H. Meier, and Ronald Melki   24. Propagation of distinct alpha-synuclein strains within human reconstructed neuronal network and associated neuronal dysfunctions Simona Gribaudo, Luc Bousset, Josquin Courte, Ronald Melki, Jean-Michel Peyrin, and Anselme L. Perrier   25. Single-particle analysis of the interaction between molecules and protein aggregated species by Dual-Color Time-Resolved Fluorescence Spectroscopy Pablo Gracia and Nunilo Cremades   26. FRAP & FRET investigation of α-synuclein fibrillization via liquid-liquid phase separation in vitro and in HeLa cells Soumik Ray, Nitu Singh, Komal Patel, G. Krishnamoorthy, and Samir K. Maji   27. Spectrally-resolved FRET microscopy of -synuclein phase-separated liquid droplets Jaladhar Mahato, Soumik Ray, Samir K. Maji, and Arindam Chowdhury   28. Combined H-N cross polarization and carbonyl detection NMR spectroscopy allows to record high-resolution, high-sensitivity spectra of alpha-synuclein in bacterial cells Juan M. Lopez   29. Identification of distinct soluble states during fibril formation using multilinear analysis of NMR diffusion data Kristine Steen Jensen, Mathias Nilsson, Mikael Akke, and Anders Malmendal   30. Structural analysis of SOD1 fibrils with mass spectrometry, limited proteolysis and atomic force microscopy (AFM) Bon-Kyung Koo and Julian Whitelegge   31. Biophysical studies of LLPS and aggregation of TDP-43 LCD W. Michael Babinchak and Witold K. Surewicz   32. A spectrophotometric turbidity assay to study Liquid-Liquid Phase Separation of UBQLN2 in vitro Peter Raymond-Smied1, Barrington Bucknor, Yiran Yang2, Tongyin Zheng, and Carlos A. Castañeda   33. An optimized SG detection method: Investigation of UBQLN2 effect on RNA-FUS interaction and SG formation Elizabeth J. Alexander and Jiou Wang   34. Neuronal puncta/aggregate formation by wild-type and mutant UBQLN2 Nathaniel Safren, Lisa M. Sharkey and Sami J. Barmada   35. In vivo analysis of a biomolecular condensate in the nervous system of C. elegans Matthew G. Andrusiak and Yishi Jin   36. FLIM-FRET investigation of heterogeneous huntingtin aggregation in HeLa cells Maria Chiara Lionetti and C.A.M La Porta   37. In vitro characterization of protein:nucleic acid liquid-liquid phase separation by microscopy methods and nanoparticle tracking analysis   Mariana J. do Amaral, Yulli M. Passos, Marcius S. Almeida, Anderson S. Pinheiro, and Yraima Cordeiro   38. Cross-seeding assay in the investigation of the amyloid core of prion fibrils Brett K.-Y. Chu, Yu-Sheng Lin, Howard C.-H. Shen, and Rita P.-Y. Chen   39 Mapping the domain structure and aggregation propensity of proteins using a Gateway plasmid vector system Beti Zaharija and Nicholas J. Bradshaw 1. Early aggregation of Amyloid-β (1-42) studied by Fluorescence Correlation Spectroscopy Mercedes Novo, Cibrán Pérez-González, Sonia Freire, and Wajih Al-Soufi   2. Preparation and investigation of crucial oligomers in the early stages of Aβ40 and Aβ42                 aggregation Bertrand Morel and Francisco Conejero-Lara   3. Preparation and fractionation of heterogeneous Aβ42 oligomers with different aggregation properties Erica W. Chen and Zhefeng Guo   4. An efficient method of expression and purification of amyloid beta (Aβ1-42) peptide from E.coli Dhiman Ghosh, Marielle Aulikki Wälti, and Roland Riek   5. Solid-state NMR structure of amyloid-β fibrils Beat H. Meier and Anja Böckmann   6. Time-resolved in situ AFM measurement of growth rates of Aβ40 fibrils Peter G. Vekilov and Peter G. Wolynes   7. Monitoring kinetics of pH-dependent aggregation and disaggregation of the Pmel17 repeat domain Dexter N. Dean and Jennifer C. Lee   8.Analysis of Tau:nucleoporin interactions by Surface Plasmon Resonance Spectroscopy Lisa Diez, Larisa E. Kapinos, Roderick Y. H. Lim, and Susanne Wegmann   9. Microfluidic chamber technology to study missorting and spreading of Tau protein in Alzheimer disease Senthilvelrajan Kaniyappan, Varun Balaji, Yipeng Wang, and Eckhard Mandelkow   10. Using FRET-based biosensor cells to study the seeding activity of tau and α-synuclein Katherine N. Maina, Caroline Smet-Nocca, and Gal Bitan   11. Functional applications of stable tau oligomers in cell biology and electrophysiology studies Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari   12. An additive-free model for tau self-assembly Youssra K Al-Hilaly, Karen E Marshall, Liisa Lutter, Luca Biasetti, Kurtis Mengham, Charles R Harrington, Wei-Feng Xue, Claude M Wischik, and Louise C Serpell   13. Cross-linking mass spectrometry analysis of metastable compact structures in intrinsically disordered proteins Dailu Chen and Lukasz A. Joachimiak   14. A validated method to prepare stable tau oligomers Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari   15. Light microscopy and dynamic light scattering to study liquid-liquid phase separation of Tau proteins in vitro Janine Hochmair, Christian Exner, Christian Betzel, Eckhard Mandelkow, and Susanne Wegmann   16. Study of tau liquid-liquid phase separation in vitro Solomiia Boyko and Witold K. Surewicz   17. Liquid-Liquid Phase Separation to study the association of proteins in solution Irving Vega and Andrew Umstead   18. Mapping phase diagram of tau-RNA LLPS under live cell coculturing conditions Yanxian Lin, Yann Fichou, Jennifer N. Rauch, Xuemei Zhang, Kenneth S. Kosik, and Songi Han   19. The role of buffers in wild-type HEWL amyloid fibril formation mechanism - a methodological approach Sandi Brudar and Barbara Hribar-Lee   20. Reproducible formation of insulin superstructures: amyloid-like fibrils, spherulites and particulates Camilla Thorlaksen, Martin Busch Neergaard, Minna Groenning, and Vito Foderà   21. CD and solid-state NMR studies of low-order oligomers of transthyretin Anvesh K. R. Dasari and Kwang Hun Lim   22. Identifying biological and biophysical features of different maturation states of α-synuclein amyloid fibrils Thomas Skamris, Bente Vestergaard, Kenneth L. Madsen, Annette E. Langkilde, and Vito Foderà   23. Preparation of α-synuclein fibril, ribbon and fibril-91 amyloid polymorphs for structural studies Luc Bousset, Ania Alik, Ana Arteni, Anja Böckmann, Beat H. Meier, and Ronald Melki   24. Propagation of distinct alpha-synuclein strains within human reconstructed neuronal network and associated neuronal dysfunctions Simona Gribaudo, Luc Bousset, Josquin Courte, Ronald Melki, Jean-Michel Peyrin, and Anselme L. Perrier   25. Single-particle analysis of the interaction between molecules and protein aggregated species by Dual-Color Time-Resolved Fluorescence Spectroscopy Pablo Gracia and Nunilo Cremades   26. FRAP & FRET investigation of α-synuclein fibrillization via liquid-liquid phase separation in vitro and in HeLa cells Soumik Ray, Nitu Singh, Komal Patel, G. Krishnamoorthy, and Samir K. Maji   27. Spectrally-resolved FRET microscopy of -synuclein phase-separated liquid droplets Jaladhar Mahato, Soumik Ray, Samir K. Maji, and Arindam Chowdhury   28. Combined H-N cross polarization and carbonyl detection NMR spectroscopy allows to record high-resolution, high-sensitivity spectra of alpha-synuclein in bacterial cells Juan M. Lopez   29. Identification of distinct soluble states during fibril formation using multilinear analysis of NMR diffusion data Kristine Steen Jensen, Mathias Nilsson, Mikael Akke, and Anders Malmendal   30. Structural analysis of SOD1 fibrils with mass spectrometry, limited proteolysis and atomic force microscopy (AFM) Bon-Kyung Koo and Julian Whitelegge   31. Biophysical studies of LLPS and aggregation of TDP-43 LCD W. Michael Babinchak and Witold K. Surewicz   32. A spectrophotometric turbidity assay to study Liquid-Liquid Phase Separation of UBQLN2 in vitro Peter Raymond-Smied1, Barrington Bucknor, Yiran Yang2, Tongyin Zheng, and Carlos A. Castañeda   33. An optimized SG detection method: Investigation of UBQLN2 effect on RNA-FUS interaction and SG formation Elizabeth J. Alexander and Jiou Wang   34. Neuronal puncta/aggregate formation by wild-type and mutant UBQLN2 Nathaniel Safren, Lisa M. Sharkey and Sami J. Barmada   35. In vivo analysis of a biomolecular condensate in the nervous system of C. elegans Matthew G. Andrusiak and Yishi Jin   36. FLIM-FRET investigation of heterogeneous huntingtin aggregation in HeLa cells Maria Chiara Lionetti and C.A.M La Porta   37. In vitro characterization of protein:nucleic acid liquid-liquid phase separation by microscopy methods and nanoparticle tracking analysis   Mariana J. do Amaral, Yulli M. Passos, Marcius S. Almeida, Anderson S. Pinheiro, and Yraima Cordeiro   38. Cross-seeding assay in the investigation of the amyloid core of prion fibrils Brett K.-Y. Chu, Yu-Sheng Lin, Howard C.-H. Shen, and Rita P.-Y. Chen   39 Mapping the domain structure and aggregation propensity of proteins using a Gateway plasmid vector system Beti Zaharija and Nicholas J. Bradshaw

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