Overview

Promoter binding analysis has largely been a laborious and inefficient process. With the advent of ChIP on chip (Chromatin Immunoprecipitation on Microarrays or Chips) the research community has been given a new, powerful and high throughput option. This book aims to not only introduce the power of this technology but specifically to provide detailed protocols as to how to perform a ChIP on chip experiment. This book will provide the reader a number of benefits: * Detailed explanations of the Chromatin Immunoprecipitation technique * Protocols for performing ChIP on chip experiments * Informatics approaches to identifying promoter regions in genomic DNA * Guidelines for analyzing the data that is obtained from a ChIP on chip experiment * Techniques for methylation analysis of CpG islands using CpG microarrays * Indications of future directions for improvements to the technology including the development of promoter specific arrays, and regulatory region arrays (enhancers and suppressors).

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9780974876566

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Reviews

'Microarrays are still predominantly used for gene expression analyses, but they have seen extensive use in genome wide (epigenetic) localization studies. This publication is a great resource for both students and researchers alike working with promotor and cpg island arrays. The chapters are highly informative, focusing on chromatin immunoprecipitation and tiling microarray analysis in both academic and commercial settings. The experimental details and data analysis methods associated with this array format are covered in a comprehensive manner'. -- Gary Hardiman, Ph.D. Director BIOGEM, Assistant Professor, Department of Medicine, University of California San Diego

Author Information

Neil A Winegarden and Mark Takahashi

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