Overview

This text provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and new tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology. It features: specific, step-by-step protocols for prokaryotic genomics and proteomics; genome mapping and DNA fingerprinting of bacteria; bacterial genetics, including methods to identify exchange and transfer genes; expression measures, genetic footprinting and microbial two-hybrid assay; protein purification with inteins; tips for data interpretation and useful websites; a troubleshooting guide; a guide to solutions; and a guide to protocols. Protocols are accompanied by notes and lab tricks and alternative methods are provided and discussed. The text is intended for: researchers working in microbiology, molecular and cell biology, biotechnology and biochemistry; biotechnology companies; and graduate and postgraduate students.

Full Product Details

Author:   Michel Blot
Publisher:   Birkhauser Verlag AG
Imprint:   Birkhauser Verlag AG
Edition:   Softcover reprint of the original 1st ed. 2003
Dimensions:   Width: 17.80cm , Height: 1.20cm , Length: 25.40cm
Weight:   0.460kg
ISBN:  

9783764365967

ISBN 10:   376436596
Pages:   208
Publication Date:   11 December 2002
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1 Physical Analysis of Chromosome Size Variation.- 2 Genetic Mapping in Salmonella enterica.- 3 Insertion Sequences as Genomic Markers.- 4 The Use of Noncoding Microsatellite Length Analysis for Bacterial Strain Typing.- 5 How to Amplify Easily, on the Bacterial Chromosome, a Desired DNA Sequence.- 6 Generalized Transduction.- 7 Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions.- 8 Genetic Footprinting for Bacterial Functional Genomics.- 9 Gene Transfer to Plants through Bacterial Vectors.- 10 Quorum Sensing: Approaches to Identify Signals and Signalling Genes in Gram-negative Bacteria.- 11 Transcriptional Profiling in Bacteria Using Microarrays.- 12 Transcriptome Analysis by Macroarrays.- 13 Prokaryotic Proteomics.- 14 Intein-mediated Protein Purification.- 15 Two-hybrid Assay in Escherichia coli K12.- Guide to Protocols.

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