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OverviewComprehensive Reference Work Detailing Basic and Advanced Information Surrounding Protein Engineering Protein engineering is a method of changing a protein sequence to achieve a desired result, such as a change in the substrate specificity or increased stability to the temperature, organic solvents, and/or extremes of pH or selectivity in catalytic reactions. Principles of Protein Engineering provides researchers the necessary fundamental knowledge about the protein engineering field and the rationale behind the techniques. Written by two experts with significant first-hand experience in the field, the work provides insight on protein synthesis, expression, structure elucidation, bioinformatics, directed evolution, stability, screening, and rational design. To aid in reader comprehension, the text is enhanced by short comments, feedback and personal anecdotes from pioneers and leaders in the field. Sample ideas covered in the work include: The fundamental knowledge of protein synthesis and expression Basic knowledge about alignment, homology modelling, dynamics of structure, and modeling enzyme kinetics The main protein engineering strategies, with a focus on directed evolution and rational design Differences between screening and selection, plus the main techniques for high-throughput screening Biochemists, biotechnologists, molecular biologists, and students in related programs of study can use Principles of Protein Engineering to attain both holistic and specific insight into the field of protein engineering and its numerous applications. Full Product DetailsAuthor: Christin Peters , Ioannis PavlidisPublisher: Wiley-VCH Verlag GmbH Imprint: Blackwell Verlag GmbH ISBN: 9783527348077ISBN 10: 3527348077 Pages: 350 Publication Date: 28 August 2024 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: Not yet available  This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release. Table of ContentsIntroduction and Retrospect to Protein Engineering PART I. Fundamentals of Proteins and Protein Expression Introduction to Proteins and Protein Conformation Protein Expression and the Selection of the Optimal Host Protein Structure Determination PART II. Bioinformatic Tools for Guiding Protein Engineering Sequence Databases Protein Modelling Enzyme Kinetics PART III. Protein Engineering Approaches/Strategies Introduction to Protein Engineering Selecting Strategy According to the Goal of the Engineering Project Directed Evolution/Random Mutagenesis Rational and Computational Design Engineering Protein Stability De Novo Design of Novel Enzymes PART IV. Screening Mutant Libraries with High-Throughput Methods Screening versus Selection Auxotrophy Development of Plate Assays for Colony Screening Photometric / Fluorometric Assays Different Compartmental ApproachesReviewsAuthor InformationIoannis Pavlidis is head of the Enzyme Technology Laboratory and Assistant Professor at the Department of Chemistry, University of Crete (GR). Having obtained his academic degrees from University of Ioannina (GR), he spent 4 years at the Department of Biotechnology and Enzyme Catalysis, University of Greifswald (DE) and later he established his own group at the University of Kassel (Germany). Christin Peters leads the Biosystems Technology group, which concentrates on enzyme cascade reactions and process optimization of large-scale biotransformations. She studied biochemistry at the University of Greifswald. Tab Content 6Author Website:Countries AvailableAll regions |
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