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OverviewMary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors. In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: life cycles of specific retroviruses and how recombinant vectors are constructed PCR techniques poliovirus vectors herpesvirus vectors syncytial assays cell lineage studies baculovirus and adenovirus vectors SV40 and EBV vectors viruses in gene transfer to eukaryotic cells. The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research. Full Product DetailsAuthor: Mary K. Collins , Mary K. L. CollinsPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1991 ed. Volume: 8 Dimensions: Width: 15.20cm , Height: 1.90cm , Length: 22.90cm Weight: 1.120kg ISBN: 9780896032996ISBN 10: 089603299 Pages: 330 Publication Date: 31 October 1991 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Out of print, replaced by POD  We will order this item for you from a manufatured on demand supplier. Table of ContentsThe Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors.- Construction of Retro viral Packaging Cell Lines.- Making High Titer Retroviral Producer Cells.- Methods of Titrating Nonselectable Recombinant Retroviruses.- Selectable Markers for Eukaryotic Cells.- Virus Genome Detection by the Polymerase Chain Reaction (PCR).- Syncytial Assays.- Neutralization of Virus.- Vesicular Stomatitis Virus Pseudotypes of Retroviruses.- Cell Lineage Studies Using Retroviruses.- Retroviral Vectors as Insertional Mutagens.- Assessment of Compounds for Anti-HIV Activity.- Analysis of Protein Factors that Interact with Adenovirus Early Promoters.- COS Cell Expression.- Papillomaviruses and Assays for Transforming Genes.- Herpes Simplex Virus Life Cycle and the Design of Viral Vectors.- Growth of Herpes Simplex Virus and Purification of Viral DNA.- Vectors Based on Epstein-Barr Virus.- Immortalization of Human B-Lymphocytes by Epstein-Barr Virus.- Vaccinia Virus as an Expression Vector.- Isolation and Handling of Recombinant Vaccinia Viruses.- Design and Construction of Poliovirus Epitope Expression Vectors.- Growth and Characterization of Poliovirus Antigen Chimeras.- Expression Vectors for the Construction of Hybrid Ty-VLPs.- Production and Purification of Hybrid Ty-VLPs.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.ReviewsI found the writing style uniformly clear and straightforward throughout...I noticed the well-organized, parallel structure of most chapters, which made them easy to follow and gave evidence of some rather careful editing. Practical Molecular Virology provides a concise, well-organized, well-written...selective overview of eukaryotic viral vectors. As an entry-level text, it surely will assist those wishing to use such vectors to make a choice among established systems. Does this book meet its objective? I believe it does. Methods books are notoriously hard to execute well, but this one succeeds by carefully defining its own niche and then occupying it. - ASM News I found the writing style uniformly clear and straightforward throughout...I noticed the well-organized, parallel structure of most chapters, which made them easy to follow and gave evidence of some rather careful editing. Practical Molecular Virology provides a concise, well-organized, well-written...selective overview of eukaryotic viral vectors. As an entry-level text, it surely will assist those wishing to use such vectors to make a choice among established systems. Does this book meet its objective? I believe it does. Methods books are notoriously hard to execute well, but this one succeeds by carefully defining its own niche and then occupying it. -ASM News Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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