Overview

This is a fully up-dated and expanded practical guide to protein structure-function relationships. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology(TM) series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Full Product Details

Author:   Christoph Kannicht
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2008
Volume:   446
Dimensions:   Width: 15.50cm , Height: 2.10cm , Length: 23.50cm
Weight:   0.623kg
ISBN:  

9781617377396

ISBN 10:   1617377392
Pages:   390
Publication Date:   19 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Disulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry.- Detection of Post-translational Modifications by Fluorescent Staining of Two-Dimensional Gels.- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.- Analysis of Tyrosine-O-Sulfation.- ?-Amidated Peptides: Approaches for Analysis.- ?-Glutamate and ?–Hydroxyaspartate in Proteins.- Lysine Hydroxylation and Cross-linking of Collagen.- Mass Spectrometric Determination of Protein Ubiquitination.- Analysis of Sumoylation.- Detection and Analysis of Protein ISGylation.- Analysis of Methylation, Acetylation, and other Modifications in Bacterial Ribosomal Proteins.- Analysis of S-Acylation of Proteins.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- 2-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.- Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB).- Separation of N-Glycans by HPLC.- Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry – detection of Lewis X Structures.- Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins.- Analysis of O-Glycosylation.- Characterization of Site-specific N-Glycosylation.- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with FluorescenceDetection and Mass Spectrometry.- Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-Linked Oligosaccharides of Recombinant Monoclonal Antibodies.- Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins.

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