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OverviewThis volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA modifications, the book guides molecular biologists toward harnessing the power of this new generation of techniques, while also introducing the data analysis skills that these high-throughput techniques require. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Post-Transcriptional Gene Regulation, Third Edition serves as a versatile resource for researchers studying post-transcriptional regulation by both introducing the most recent techniques and providing a comprehensive guide to their implementation. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. Full Product DetailsAuthor: Erik DassiPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 3rd ed. 2022 Volume: 2404 Weight: 0.810kg ISBN: 9781071618530ISBN 10: 1071618539 Pages: 411 Publication Date: 27 October 2022 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsPart I: Bioinformatics 1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression Eliana Destefanis and Erik Dassi 2. Predicting RNA Secondary Structure Using In Vitro and In Vivo Data Riccardo Delli Ponti and Gian Gaetano Tartaglia 3. RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment Inbal Paz, Amir Argoetti, Noa Cohen, Niv Even, and Yael Mandel-Gutfreund Part II: Expression Studies 4. Analysis of mRNA Translation by Polysome Profiling Anne Cammas, Pauline Herviou, Leïla Dumas, and Stefania Millevoi 5. Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling Alexander L. Cope, Sangeevan Vellappan, John S. Favate, Kyle S. Skalenko, Srujana S. Yadavalli, and Premal Shah 6. Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq Sahil Sharma and Cynthia M. Sharma 7. Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems Katharina G. Wandera and Chase L. Beisel Part III: Interactomics 8. Studying RNP Composition with RIP Annalisa Rossi and Alberto Inga 9. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites Charles Danan, Sudhir Manickavel, and Markus Hafner 10. A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq Sudeep Sahadevan, Thileepan Sekaran, and Thomas Schwarz 11. Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH) Andrea Zeni, Margherita Grasso, and Michela A. Denti 12. Identifying the Protein Interactomes of Target RNAs Using HyPR-MS Katherine B. Henke, Rachel M. Miller, Rachel A. Knoener, Mark Scalf, Michele Spiniello, and Lloyd M. Smith Part IV: The RNA Lifecycle 13. Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization Ankita Arora, Raeann Goering, Pedro Tirado Velez, and J. Matthew Taliaferro 14. Single-Molecule RNA Imaging Using Mango II Arrays Adam D. Cawte, Haruki Iino, Peter J. Unrau, and David S. Rueda 15. Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq Yeonui Kwak and Hojoong Kwak 16. In Vivo RNA Structure Probing with DMS-MaPseq Paromita Gupta and Silvia Rouskin 17. Transcriptome-Wide Profiling of RNA Stability Nina Fasching, Jan Petržílek, Niko Popitsch, Veronika Herzog, and Stefan L. Ameres 18. High-Throughput Quantitation of Yeast uORF Regulatory Impacts Using FACS-uORF Gemma E. May and C. Joel McManus Part V: RNA Modifications 19. m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine Devi Prasad Bhattarai and Francesca Aguilo 20. Detecting m6A with In Vitro DART-Seq Matthew Tegowski1, Huanyu Zhu, and Kate D. Meyer 21. Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing Tennille Sibbritt, Ulrike Schumann, Andrew Shafik, Marco Guarnacci, Susan J. Clark, and Thomas Preiss 22. Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq Yinzhou Zhu, Christopher L. Holley, and Gordon G. CarmichaelReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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