Overview

Full Product Details

Author:   Alexei V. Tulin
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2023
Volume:   2609
Weight:   1.063kg
ISBN:  

9781071628904

ISBN 10:   1071628909
Pages:   446
Publication Date:   15 December 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Detection and Quantification of pADPr   1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis             Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung   2. Detecting and Quantifying pADPr In Vivo             Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark   3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage             Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol   4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose)             Johannes Rudolph and Karolin Luger   5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers             Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus   6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo             Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa   7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy             Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger   8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery             Johannes Gregor Matthias Rack and Ivan Ahel   Part II: Identification of Protein Targets   9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies             Helen Dauben and Ivan Matic   10. A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins             Dongsheng Yao, Heba Ahmed, and Junqi Song   11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells             Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus   Part III: Functional Analysis   12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer             Nura Lutfi, Carlos Martínez, and José Yélamos   13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death             Hannah K. Hall and David W. Koh   14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function             Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai   15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry             Holda A. Anagho, Jonas D. Elsborg, Ivo A. Hendriks, Sara C. Buch-Larsen, and Michael L. Nielsen   16. Cytological Approaches to Visualize Intracellular Dynamics of RNA Binding Proteins at Active Genes in Drosophila             Anand K. Singh and Subhash C. Lakhotia   Part IV: Poly(ADP-Ribosyl)ating Pathway in Chromatin and Gene Expression   17. Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin             Guillaume Bordet, Gbolahan Bamgbose, Sayem H. Bhuiyam, Sarah Johnson, and Alexei V. Tulin   18. Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates             Hejer Dhahri, Elena Matveeva, and Yvonne Fondufe-Mittendorf   19. Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells             Peter Makhov, Rushaniya Fazliyeva, Antonio Tufano, Robert G. Uzzo, and Vladimir M. Kolenko   Part V: Using Animal Models   20. Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo             Guillaume Bordet and Alexei V. Tulin   21. Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System             Yaroslava Karpova and Alexei V. Tulin   22. TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice             Yaroslava Karpova and Alexei V. Tulin   Part VI: Designing and Testing PARP-1 Inhibitors   23. Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells             Yaroslava Karpova, Danping Guo, and Alexei V. Tulin   24. Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System             Anna K. Duell, Daniel J. Sanderson, and Michael S. Cohen   Part VII: Targeting the Understudied Components of pADPr Pathway   25. Purification of Recombinant Human PARG and Activity Assays             Jean-Christophe Amé and Françoise Dantzer   26. Purification of Recombinant Human PARP-3             Jean-Christophe Amé, Leonel Nguekeu-Zebase, Daisy Harwood, Zuleyha Yildirim, Lisa Roegel, Agathe Boos, and Françoise Dantzer

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions