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OverviewThis volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field. Full Product DetailsAuthor: Javier MartínPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of the original 1st ed. 2016 Volume: 1387 Dimensions: Width: 17.80cm , Height: 1.90cm , Length: 25.40cm Weight: 0.677kg ISBN: 9781493980130ISBN 10: 1493980130 Pages: 341 Publication Date: 14 April 2018 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand ![]() We will order this item for you from a manufactured on demand supplier. Table of ContentsAn Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication.- Poliovirus Laboratory Based Surveillance: An Overview.- Isolation and Characterisation of Enteroviruses from Clinical Samples.- Isolation and Characterisation of Poliovirus in Cell Culture Systems.- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol.- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays.- A Transgenic Mouse Model of Poliomyelitis.- Standardized Methods for Detection of Poliovirus Antibodies.- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR.- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative.- Phylogenetic Analysis of Poliovirus Sequences.- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA.- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses.- Methods to Monitor Molecular Consistency of Oral Polio Vaccine.- Methods for the Quality Control of Inactivated Poliovirus Vaccines.- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.- Identification and Analysis of Antiviral Compounds against Poliovirus.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |