Overview

For both volumes: A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods are to the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Full Product Details

Author:   Jonathan M. Gibbins ,  Martyn P. Mahaut-Smith
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   273
Dimensions:   Width: 15.20cm , Height: 3.40cm , Length: 22.90cm
Weight:   1.054kg
ISBN:  

9781588290113

ISBN 10:   1588290115
Pages:   504
Publication Date:   13 August 2004
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Perspectives.- Signaling Receptors on Platelets and Megakaryocytes.- Manipulation of Gene Expression in Megakaryocytes.- In Vitro Changes of Platelet Parameters.- Pharmacological Approaches to Studying Platelet Function.- Inhibitors of Cellular Signaling Targets.- Signaling Techniques.- Ligand-Binding Assays for Collagen.- Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines.- Ligand-Binding Assays.- Techniques for Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting.- Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation.- Protein Tyrosine Phosphatases.- The Study of Serine-Threonine Kinases.- Phosphoinositides.- Isolation and Analysis of Platelet Lipid Rafts.- Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures.- Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes.- Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte.- Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets.- Nitric Oxide Signaling in Platelets.- Snake Venom Toxins Affecting Platelet Function.- Peptide Synthesis in the Study of Collagen-Platelet Interactions.- Platelet Permeabilization.- Molecular and Post-Genomic Techniques.- Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells.- Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes.- GFP Fusion Proteins to Study Signaling in Live Cells.- Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics.- Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes.- Platelet Receptor Structures and Polymorphisms.- Gene Array Technology and the Study of Platelets and Megakaryocytes.

Reviews

...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes. - Veterinary Pathology

.,. thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes. - Veterinary Pathology

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