Overview

For both volumes: A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods are to the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Full Product Details

Author:   Jonathan M. Gibbins ,  Martyn P. Mahaut-Smith
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   272
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.50cm
Weight:   0.857kg
ISBN:  

9781588291011

ISBN 10:   1588291014
Pages:   385
Publication Date:   01 July 2004
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Platelet Functional Assays.- Effects of Anticoagulants Used During Blood Collection on Human Platelet Function.- Preparation of Washed Platelet Suspensions From Human and Rodent Blood.- Platelet Counting.- Electron Microscopy Methods for Studying Platelet Structure and Function.- Platelet Aggregation.- Platelet Aggregation in Whole Blood.- Secretion From Dense Granules.- Platelet Dense-Granule Secretion.- Analysis of the Releasable Nucleotides of Platelets.- Studies of Secretion Using Permeabilized Platelets.- Measurement of Platelet Arachidonic Acid Metabolism.- Measurement of the Platelet Procoagulant Response.- Platelet Adhesion Assays Performed Under Static Conditions.- Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns.- Techniques to Examine Platelet Adhesive Interactions Under Flow.- In Vivo Models of Platelet Function and Thrombosis.- Adhesion Between Platelets and Leukocytes or Endothelial Cells.- In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100®.- Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation.- Flow-Cytometric Analysis of Mouse Platelet Function.- Platelet Microparticles.- Megakaryocyte Functional Assays.- Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation.- Isolation and Culture of Megakaryocyte Precursors.- Assays of Megakaryocyte Development.- Assays of Megakaryocyte Development.- Thrombopoietin Bioassay.- Culture of Megakaryocytic Cell Lines.

Reviews

...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes. - Veterinary Pathology

.,. thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes. - Veterinary Pathology

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