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OverviewSignal transduction is the fundamental mechanism for regulation of cellular activities by environmental cues and regulatory signals, and is particularly important for plants, whose survival requires proper physiological and developmental responses to the environmental changes. Much progress has been made recently in the plant signal transduction research field thanks to the development of diverse techniques which are covered in Plant Signalling Networks: Methods and Protocols. These include advanced research methods such as proteomics and mass spectrometry methods for studying protein modification, biochemical and cell biological tools for studying protein-protein interactions, genomic techniques for dissecting protein-DNA interaction and transcription networks, and computation methods that integrate molecular network into plant developmental processes. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Plant Signalling Networks: Methods and Protocols presents well-honed methodologies for a wide range of research approaches including genetics, proteomics, biochemical, cell biological, and computational approaches, and seeks to serve both professionals and novices with a comprehensive understanding of complex signaling networks in plants. Full Product DetailsAuthor: Zhi-Yong Wang , Zhenbiao Yang , Zhi-Yong Wang, DrPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of the original 1st ed. 2012 Volume: 876 Dimensions: Width: 17.80cm , Height: 1.30cm , Length: 25.40cm Weight: 0.641kg ISBN: 9781493961696ISBN 10: 1493961691 Pages: 230 Publication Date: 23 August 2016 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsExperimental Analysis of Receptor Kinase Phosphorylation.- Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies.- Identification of O-GlcNAc Modified Proteins from Arabidopsis.- Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE).- Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE).- Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana.- Small-Molecule Dissection of Brassinosteroid (BR) Signaling.- A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination.- Activation Tagging.- Rho GTPase Activity Analysis in Plant Cells.- Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy.- In vivo Ubiquitination Assay by Agroinfiltration.- In Vitro Protein Ubiquitination Assay.- Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq).- Smart Pooling of mRNA Samples for Efficient Transcript Profiling.- Transient Expression Assays for Quantifying Signaling Output.- Genome-Wide Profiling of Uncapped mRNA.- Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches.ReviewsFrom the reviews: This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user. (Peter Schroeder, Plant Science Bulletin, Vol. 59 (1), 2013) From the reviews: This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user. (Peter Schroder, Plant Science Bulletin, Vol. 59 (1), 2013) From the reviews: This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user. (Peter Schroder, Plant Science Bulletin, Vol. 59 (1), 2013) Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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