Overview

The Plant Pathology Laboratory Manual (2025) is a comprehensive practical guide designed for undergraduate agricultural science students. It introduces the fundamental concepts of plant diseases, including their causes, classification, economic impacts, and diagnostic techniques. The manual provides step-by-step laboratory procedures for identifying plant pathogens such as fungi, bacteria, viruses, and nematodes.It covers essential laboratory practices like sterilization, disinfection, equipment handling, safety rules, and proper labeling through ensuring students to develop aseptic techniques. Also, detailed practical sessions guide learners through smear preparation, microscopic observation, culture media preparation, isolation of phytopathogens from soil and plant tissues, preservation of diseased specimens, pathogenicity testing, Koch's postulates, and detection of seed-borne pathogens.Overall, the manual equips learners with the knowledge and hands-on skills required to diagnose plant diseases effectively and perform basic research in plant pathology laboratories.

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