Overview

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Full Product Details

Author:   Yiping Qi
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1917
Weight:   0.943kg
ISBN:  

9781493989904

ISBN 10:   1493989901
Pages:   367
Publication Date:   05 January 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Plant DNA Repair Pathways and their Applications in Genome Editing.- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method.- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations.- Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9.- A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing.- A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants.- CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation.- Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9.- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9.- An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize.- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants.- Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9.- Application of CRISPR/Cas9-Mediated Gene Editing in Tomato.- Genome Editing in Potato with CRISPR/Cas9.- Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells.- Genome Editing in Soybean with CRISPR/Cas9.- Genome Editing in Citrus Tree with CRISPR/Cas9.- Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems.- Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1.- Targeted Mutagenesis using FnCpf1 in Tobacco.- Gene Replacement by Intron Targeting with CRISPR-Cas9.- Targeted Base Editing with CRISPR-Deaminase in Tomato.- Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System.- Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat.- DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce.- An Automated Protoplast Transformation System.

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