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Author:   Heddwyn Jones
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1996 ed.
Volume:   49
Dimensions:   Width: 15.20cm , Height: 2.80cm , Length: 22.90cm
Weight:   0.001kg
ISBN:  

9780896033214

ISBN 10:   089603321
Pages:   466
Publication Date:   27 September 1995
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Spiral bound
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Agrobacterium-Mediated Transformation.- Tools for Expressing Foreign Genes in Plants.- of Cloning Plasmids into Agrobacterium tumefaciens.- Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco.- Agobacterium rhizogenes as a Vector for Transforming Higher Plants.- Agrobacterium-Mediated Transformation of Arabidopsis thaliana.- Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues.- Direct Gene Transfer.- Stable Transformation of Barley via Direct DNA Uptake.- Gene Transfer into Plant Protoplasts by Electroporation.- Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues.- Use of Reporter Genes.- The ?-Glucuronidase (gus) Reporter Gene System.- Chloramphenicol Acetyl Transferase Assay.- NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants.- Study of Gene Organization by Southern Blotting and Inverse PCR.- Gene Characterization by Southern Analysis.- Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification.- RNA Techniques for Studying Gene Expression.- Isolation of Whole Cell (Total) RNA.- Poly(A)+RNA Isolation.- In Vitro Translation.- Northern Analysis and Nucleic Acid Probes.- Nuclear “Run-On” Transcription Assays.- RNase A/T1 Protection Assay.- Primer Extension Assay.- Applications of RT-PCR.- In Vitro Transcription of Class II Promoters in Higher Plants.- Analysis of Plant Gene Expression by Reverse Transcription-PCR.- In Situ Hybridization to Plant Tissue Sections.- Xenopus Oocytes as a Heterologous Expression System.- Heterologous Expression in Yeast.- Techniques for Studying Chloroplast Gene Expression.- The Isolation of Intact Chloroplasts.- In Vitro Protein Import by IsolatedChloroplasts.- Targeting of Foreign Proteins to the Chloroplast.- Techniques for Studying Mitochondrial Gene Expression.- Isolation of Mitochondria.- Mitochondrial Nucleic Acid Purification and Analysis.- In organello Protein Synthesis.- Immunological Detection of Proteins.- Separation of Plant Proteins by Electrophoresis.- Western Blotting Analysis.- ELISA Detection of Foreign Proteins.- Immunocytochemical Localization of Proteins.

Reviews

The chapters were written by outstanding scientists and [the book] can be recommended to any laboratory working with plants, providing tips and alternatives in case of problems. -Journal of Plant Physiology

The chapters were written by outstanding scientists and [the book] can be recommended to any laboratory working with plants, providing tips and alternatives in case of problems. - Journal of Plant Physiology

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