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OverviewThis volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology. Chapters focus on phenotypic screening, the use of human cell models, microscopic approaches, assays to measure fat accumulation in C. elegans, the threat response in zebrafish, and protein-protein interactions in plant growth and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phenotypic Screening: Methods and Protocols aims to make phenotypic screening approaches more accessible to a wide array of researchers throughout the academic and biotech communities. Full Product DetailsAuthor: Bridget WagnerPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of the original 1st ed. 2018 Volume: 1787 Weight: 0.559kg ISBN: 9781493993031ISBN 10: 1493993038 Pages: 280 Publication Date: 10 December 2019 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Nutrient Stress Small-Molecule Screening Platform for Escherichia coli Sara S. El Zahed, Garima Kumar, Madeline Tong, and Eric D. Brown 2. Validation and Development of an Escherichia coli Riboflavin Pathway Phenotypic Screen Hit as a Small-molecule Ligand of the Flavin Mononucleotide Riboswitch Carl J. Balibar, Artjohn Villafania, Christopher M. Barbieri, Nick Murgolo, Terry Roemer, Hao Wang, and John A. Howe 3. Phenotypic Screening of Small Molecules with Anti-malarial Activity for Three Different Parasitic Life Stages Nobutaka Kato, Sandra March, Sangeeta N Bhatia, and Matthias Marti 4. Phenotypic Screening for Inhibitors of a Mutant Thrombopoietin Receptor Anna Ngo, Ann Koay, Christian Pecquet, Carmen C. Diaconu, David A. Jenkins, Andrew K. Shiau, Stefan N. Constantinescu, and Meng Ling Choong 5. A Cell-based Assay for Mitotic Spindle Orientation Elina Glaubke and Holger Bastians 6. Isolation of Skeletal Muscle Stem Cells for Phenotypic Screens for Modulators of Proliferation Andrew N. Billin and Aaron C. Hinken 7. Cell-based Methods to Identify Inducers of Human Pancreatic Beta-cell Proliferation Courtney A. Ackeifi, Ethan A. Swartz, and Peng Wang 8. High-content Imaging Phenotypic Screen for Neurogenesis Using Primary Neural Progenitor Cells Li Sharon Wu and Jingjun Li 9. Discovery of Modulators of Adipocyte Physiology Using Fully Functionalized Fragments Andrea Galmozzi, Christopher G. Parker, Bernard P. Kok, Benjamin F. Cravatt, and Enrique Saez 10. The Ancient Genetic Networks of Obesity: Whole-animal Automated Screening for Conserved Fat Regulators Wenfan Ke, Anna Drangowska-Way, Daniel Katz, Karsten Siller, and Eyleen J. O’Rourke 11. Modulation of Threat Response in Larval Zebrafish Andrew J. Rennekamp 12. A PLA-iRoCS Pipeline for the Localization of Protein-Protein Interactions in situ Taras Pasternak, William Teale, Thorsten Falk, Benedetto Ruperti, and Klaus Palme 13. High-Dimensional Profiling: The Theta Comparative Cell Scoring Method Scott J Warchal, John C Dawson, and Neil O Carragher 14. Accessing the Open PHACTS Discovery Platform with Workflow Tools Daniela Digles, Andrei Caracoti, and Edgar Jacoby 15. Quantitative Prioritization of Tool Compounds for Phenotypic Screening Yuan Wang and Jeremy L. Jenkins 16. A Quantitative Systems Pharmacology Approach to Infer Pathways Involved in Complex Disease Phenotypes Mark E. Schurdak, Fen Pei, Timothy R. Lezon, Diane Carlisle, Robert Friedlander, D. Lansing Taylor, and Andrew M. Stern 17. Phenotype-based High-content Screening using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells Young-Hee Shin and Seung Bum Park 18. Developing High-throughput Electrophysiological Assays for Phenotypic Screening Jen Q. Pan, David Baez, Andrew Allen, and Hao-Ran Wang 19. Testing Susceptibility of Patient Derived Organoid Cultures to Therapies: Pharmacotyping Richard A. Burkhart, Lindsey A. Baker, and Hervé Tiriac 20. Techniques to Enable 1536-well Phenotypic Screening Sinéad Knight, Helen Plant, Lisa McWilliams, and Mark WigglesworthReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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