Overview

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies. PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.

Full Product Details

Author:   Gerard Morel (Universite Claude Bernard, France) ,  Mireille Raccurt (Universite Claude Bernard, Villeurbanne Cedex, France)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Volume:   6
Dimensions:   Width: 17.80cm , Height: 2.90cm , Length: 25.40cm
Weight:   1.014kg
ISBN:  

9780849300417

ISBN 10:   084930041
Pages:   444
Publication Date:   27 September 2002
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

General Introduction Chapter 1 - General Principles Chapter 2 - Preparation of Samples Chapter 3 - Pretreatments Chapter 4 - Reverse Transcription (RT) Chapter 5 - Polymerase Chain Reation (PCR) Chapter 6 - Hybridization Chapter 7 - Revelation Chapter 8 - Electron Microscopy Chapter 9 - Controls and Problems Chapter 10 - Typical Protocols Chapter 11 - Examples of Observations

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Author Information

Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.

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