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OverviewMolecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects.PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression, differential display cloning, cloning and library screening by PCR, genotype and polymorphism analysis of drug and toxicant metabolizing enzymes, basic, non-PCR based molecular biology methods. PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential. Full Product DetailsAuthor: John P. Vanden Heuvel , Edward J. Massaro (Consultant, Morrisville, North Carolina, USA) , Edward J. Massaro (Consultant, Morrisville, North Carolina, USA) , Gary PittmanPublisher: Taylor & Francis Inc Imprint: CRC Press Inc Dimensions: Width: 15.60cm , Height: 1.40cm , Length: 23.40cm Weight: 0.376kg ISBN: 9780849333446ISBN 10: 084933344 Pages: 252 Publication Date: 24 September 1997 Audience: Professional and scholarly , Professional and scholarly , Professional & Vocational , Postgraduate, Research & Scholarly Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsChapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4 . Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6 . General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I — List of Suppliers -- Appendix II — Companies, Online Catalogs, and Internet Resources -- Index.ReviewsAuthor InformationVanden Heuvel, John P. Tab Content 6Author Website:Countries AvailableAll regions |
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