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OverviewThis manual introduces the complex world of PCR by beginning at an accessible level and then moving to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced and then the basic aspects of the technique are explained by exploring important issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a range of PCR and amplification techniques are presented for cloning, sequencing, mutagenesis, library construction and screening, exon trapping, differential display, and expression, and these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, in situ PCR, and litigation-mediated PCR. Each protocol is augmented by analysis and troubleshooting sections and complete references. Full Product DetailsAuthor: Carl W. Dieffenbach , Gabriela S. DvekslerPublisher: Cold Spring Harbor Laboratory Press,U.S. Imprint: Cold Spring Harbor Laboratory Press,U.S. Edition: illustrated edition ISBN: 9780879694487ISBN 10: 0879694483 Pages: 714 Publication Date: 01 January 1995 Audience: College/higher education , Professional and scholarly , Postgraduate, Research & Scholarly , Professional & Vocational Format: Spiral bound Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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