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OverviewDistinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs. Full Product DetailsAuthor: Richard WhitePublisher: Humana Press Inc. Imprint: Humana Press Inc. Volume: v. 67 Dimensions: Width: 22.90cm , Height: 3.00cm , Length: 15.20cm Weight: 0.910kg ISBN: 9780896033436ISBN 10: 0896033430 Pages: 512 Publication Date: 01 October 1996 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Spiral bound Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsReviewsThis volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis. - Biochemical Education Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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