|
|
|||
|
||||
OverviewPCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Full Product DetailsAuthor: Bing-Yuan Chen , Harry W. JanesPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2nd ed. 2002 Volume: v. 192 Dimensions: Width: 15.50cm , Height: 2.30cm , Length: 23.50cm Weight: 0.635kg ISBN: 9780896039735ISBN 10: 0896039730 Pages: 453 Publication Date: 01 April 2002 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Unknown Availability: Out of print, replaced by POD We will order this item for you from a manufatured on demand supplier. Table of ContentsReviewsThis hardcover book is packed full of detailed protocols and methodological articles on various aspects of PCR. The fact that the book was taken out of my hands by a colleague, to read up on touch-down PCR, as soon as I received it for review, attest to the book's usefulness. I will have to chain this book to my bookshelf, to make sure it does not disappear quickly-or I may consider buying a couple for the laboratory. -Plant Science Review of the first edition. This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis.-Biochemical Education ...the book provides a valuable reference source for PCR-related technologies and protocols for students, postdocs and technicians. - Heredity Review of the first edition. This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis. - Biochemical Education Author InformationTab Content 6Author Website:Countries AvailableAll regions |