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OverviewPCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis. Full Product DetailsAuthor: M. J. McPherson (Department of Biochemistry) , B. D. Hames (Department of Biochemistry, both at the University of Leeds) , G.R. Taylor (Regional DNA Laboratory, Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 150 Dimensions: Width: 15.60cm , Height: 2.00cm , Length: 23.40cm Weight: 0.533kg ISBN: 9780199634248ISBN 10: 0199634246 Pages: 358 Publication Date: 01 June 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: Awaiting stock  Table of Contents1: Optimizing PCR 2: Use of specialty phosphoramidites in PCR 3: Chemical methods for 5' non-isotopic labelling of PCR probes and primers 4: Solid-phase PCR 5: Solid-phase sequencing of PCR products 6: cDNA cloning by RT - PCR 7: Quantification of DNA and RNA by PCR 8: PCR MIMICS: competitive DNA fragments for use in quantitative PCR 9: In vitro expression of proteins from PCR products 10: PCR-based approaches to human genome mapping 11: Fingerprinting of DNA and RNA using arbitrarily primed PCR 12: Mutational analysis 13: Mutational analysis: new mutations 14: Linear amplification for the in virto study of ligand/DNA interactions 15: Ligand-mediated PCRReviews`This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire.' Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6 This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6 Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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