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OverviewChannels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques. Full Product DetailsAuthor: Yasunobu OkadaPublisher: Springer Verlag, Japan Imprint: Springer Verlag, Japan Edition: Softcover reprint of the original 1st ed. 2012 Weight: 0.854kg ISBN: 9784431561057ISBN 10: 4431561056 Pages: 439 Publication Date: 23 August 2016 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsCh. 1 Prologue: The Ion Channel Ch. 2 Patch-Clamp Techniques: General Remarks Ch. 3 Whole-Cell Patch Method Ch. 4 Perforated Whole-Cell Patch-Clamp Technique: A User’s Guide Ch. 5 Methods for Processing and Analyzing Single-Channel Data Ch. 6 Channel Noise Ch. 7 Slice Patch Clamp Ch. 8 Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms Ch. 9 Analysis of Synaptic Plasticity with the Slice Patch-Clamp Recording Technique Ch. 10 Patch-Clamp Recordings from Neuronal Dendrites Ch. 11 In Vivo Blind Patch-Clamp Recording Technique Ch. 12 Two-Photon Targeted Patch-Clamp Recordings in Vivo Ch. 13 Transporter Current Measurements Ch. 14 Giant Patch and Macro Patch Ch. 15 Pipette Perfusion Technique Ch. 16 Planar Lipid Bilayer Method for Studying Channel Molecules Ch. 17 Patch-Clamp Capacitance Measurements Ch. 18 Brief Guide to Patch-Clamp Current Measurements in Organelle Membranes Ch. 19 Role of Ion Channels in Plants Ch. 20 Automated Patch Clamping Ch. 21 Patch-Clamp Biosensor Method Ch. 22 Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature Ch. 23 Heterologous Expression Systems and Analyses of Ion Channels Ch. 24 Patch-Clamp and Single-Cell Reverse Transcription-Polymerase Chain Reaction/Microarray Analysis Ch. 25 Smart-Patch Technique Ch. 26 Ion Channel Pore Sizing in Patch-Clamp Experiments Ch. 27 Digital Recordings of Patch-Clamp Data Ch. 28 Solutions for Patch-Clamp ExperimentsReviewsFrom the reviews: This book is the most current single `how to' manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. ... The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons. (Joseph J. Grenier, Amazon.com, May, 2014) From the reviews: This book is the most current single `how to' manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. ... The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons. (Joseph J. Grenier, Amazon.com, May, 2014) From the reviews: “This book is the most current single ‘how to’ manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. … The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons.” (Joseph J. Grenier, Amazon.com, May, 2014) Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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