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OverviewThis detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methodsand Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines. Full Product DetailsAuthor: Arne GennerichPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 2nd ed. 2022 Volume: 2478 Weight: 1.630kg ISBN: 9781071622285ISBN 10: 1071622285 Pages: 757 Publication Date: 06 September 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsPart I: Historic Views on the Invention of Lasers and Optical Tweezers 1. The Invention of the Laser Nick Taylor 2. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation Matthias D. Koch and Joshua W. Shaevitz Part II: Technical Advances 3. Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes Swathi Sudhakar, Viktoria Wedler, Pasupathi Rajendran, and Erik Schäffer 4. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics Xiang Gao, James T. Inman, and Michelle D. Wang 5. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System Anna E.C. Meijering, Julia A.M. Bakx, Tianlong Man, Iddo Heller, Gijs J.L. Wuite, and Erwin J.G. Peterman 6. One-Dimensional STED Microscopy in Optical Tweezers Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, and Iddo Heller 7. Temperature Quantification and Temperature Control in Optical Tweezers Joost J. Geldhof, Agata M. Malinowska, Gijs J.L. Wuite, Erwin J.G. Peterman, and Iddo Heller 8. High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock Part III: DNA, DNA Motors and DNA-Protein Interactions 9. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers Graeme A. King, Dian Spakman, Erwin J.G. Peterman, and Gijs J.L Wuite 10. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay Arnulf M.K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins 11. Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers Rachel Leicher and Shixin Liu 12. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun 13. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda Part IV: Protein (Un)Folding 14. Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante 15. Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone Katarzyna Tych and Matthias Rief 16. Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments Kevin Maciuba and Christian M. Kaiser 17. Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers Huaizhou Jin, Jinghua Ge, Jie Yang, and Yongli Zhang 18. Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes Lihui Bai, Mira Malmosi, Lydia Good, and Rodrigo A. Maillard Part V: Cytoskeletal Motors and Proteins 19. High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, and Marco Capitanio 20. Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap 21. Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A Lu Rao and Arne Gennerich 22. Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins Suvranta K. Tripathy, Vladimir M. Demidov, Ivan V. Gonchar, Shaowen Wu, Fazly I. Ataullakhanov, and Ekaterina L. Grishchuk Part VI: Function and Mechanical Properties of Filaments 23. Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips Lucas E. Murray, Haein Kim, Luke M. Rice, and Charles L. Asbury 24. Mechanics of Single Vimentin Intermediate Filaments under Load Anna V. Schepers, Julia Kraxner, Charlotta Lorenz, and Sarah Köster 25. Quantifying the Interaction Strength between Biopolymers Charlotta Lorenz, Anna V. Schepers, and Sarah Köster Part VII: Mechanosensing of Membrane Channels 26. Measuring αβ T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging Hannah M. Stephens, Kristine N. Brazin, Robert J. Mallis, Yinnian Feng, Debasis Banik, Ellis L. Reinherz, and Matthew J. LangReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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