Overview

The steroid / nuclear receptor superfamily is a large and growing group of transcription factors that are studied by a large and varied number of basic and clinical researchers. The first two chapters describe the evolutionary biology of the superfamily and explain how to clone and characterize new receptors. Chapter 3 shows how to identify the ligands of novel receptors and chapter 4 explains the kinetic analysis of receptor interactions. In chapter 5, the reader is guided through the functional characterization of coactivators using microinjection. The next section covers receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. Chapter 9 describes the in vitro assembly of Hsp90 complexes and chapter 10 explains yeast as a model system for looking at receptor function. The final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a comprehensive guide to studying members of the superfamily and will be invaluable to all researchers old and new.

Full Product Details

Author:   Didier Picard (Department of Cellular Biology, University of Geneva)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   207
Dimensions:   Width: 16.30cm , Height: 1.70cm , Length: 21.90cm
Weight:   0.445kg
ISBN:  

9780199637423

ISBN 10:   0199637423
Pages:   304
Publication Date:   08 July 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Preface Table of Contents List of Contributors 1: Evolutionary biology of the nuclear receptor superfamily 2: Initial characterization of new orphan receptors 3: Adopting orphans: finding ligands 4: Kinetic analysis of nuclear receptor interactions 5: Functional characterization of coactivators using mammalian cell microinjection 6: Analysis of steroid/nuclear receptor phosphorylation 7: Ligand- and cofactor-regulated transcription with chromatin templates 8: Hormone resistance syndromes 9: In Vitro assembly of steroid receptor - Hsp90 complexes 10: Yeast as a system for the study of nuclear receptor function 11: Regulation of heterologus proteins by fusion to a hormone binding domain List of non-standard Abbreviations List of suppliers Appendices

Reviews

... An authoritative and wide-ranging 'methods' book in a distinguished series Aslib Book Guide Dr Picard has done an excellent job in bringing together a range of exciting techniques that are currently being used to futher our understanding of this fascinating and ever growing family of 'Nuclear Recptors Cell Biology International

The editor more than meets his goal by providing technical information both for scientists interested in nuclear receptors and for others interested in the analysis of protein-mediated cellular processes. An exceptionally fine feature of this work is the layout of each specific protocol in an easy to follow box format. These protocols range from the most simplistic, such as preparation of coverslips for microscopy to detailed descriptions of the assays used to analyze ligand binding by receptors. This would be a useful addition to any cell biology laboratory. The wealth of useful techniques described should prove a useful starting point for anyone interested in pursuing the analysis of nuclear receptors as well as other cellular functions. -- Doody's<br>

... An authoritative and wide-ranging 'methods' book in a distinguished series Aslib Book Guide Dr Picard has done an excellent job in bringing together a range of exciting techniques that are currently being used to futher our understanding of this fascinating and ever growing family of 'Nuclear Recptors Cell Biology International

<br> The editor more than meets his goal by providing technical information both for scientists interested in nuclear receptors and for others interested in the analysis of protein-mediated cellular processes. An exceptionally fine feature of this work is the layout of each specific protocol in an easy to follow box format. These protocols range from the most simplistic, such as preparation of coverslips for microscopy to detailed descriptions of the assays used to analyze ligand binding by receptors. This would be a useful addition to any cell biology laboratory. The wealth of useful techniques described should prove a useful starting point for anyone interested in pursuing the analysis of nuclear receptors as well as other cellular functions. -- Doody's<p><br>

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions