Overview

This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Non-Ribosomal Peptide Biosynthesis and Engineering: Methods and Protocols aims to feature methods that will be beneficial to new researchers, and those wanting to adopt new methodologies into their research.

Full Product Details

Author:   Michael Burkart ,  Fumihiro Ishikawa
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2670
Weight:   0.831kg
ISBN:  

9781071632130

ISBN 10:   1071632132
Pages:   321
Publication Date:   16 May 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I Background and Overview 1. The Assembly-line Enzymology of Nonribosomal Peptide Biosynthesis Chitose Maruyama and Yoshimitsu Hamano   2. Structural Studies of Modular Nonribosomal Peptide Synthetases Ketan D. Patel, Syed Fardin Ahmed, Monica R. MacDonald, and Andrew M. Gulick   Part II In Vivo and In Vitro Methods 3. Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent FAS-II Desirae A. Mellor, Javier O. Sanlley, and Michael D. Burkart   4. Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal Peptide Synthetases Fumihiro Ishikawa and Genzoh Tanabe   5. In Vitro Biochemical Characterization of Excised Macrocyclizing Thioesterase Domains from Non-Ribosomal Peptide Synthetases Jordan T. Brazeau-Henrie, André R. Paquette, and Christopher N. Boddy   6. Chemo-Enzymatic Synthesis of Non-Ribosomal Macrolactams by a Penicillin-binding Protein-type Thioesterase Masakazu Kobayashi, Kei Fujita, Kenichi Matsuda, and Toshiyuki Wakimoto   7. Directed Evolution of the BpsA Carrier Protein Domain for Recognition by Non-cognate 4’-Phosphopantetheinyl Transferases to Enable Inhibitor Screening Alistair S. Brown, Jeremy G. Owen, and David F. Ackerley   8. Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Crosslinking Analysis with Genetic Code Expansion Julia Diecker, Wolfgang Dörner, Jennifer Rüschenbaum, and Henning D. Mootz   9. A Chemoenzymatic Approach to Investigate Cytochrome P450 Crosslinking in Glycopeptide Antibiotic Biosynthesis Y. T. Candace Ho, Yongwei Zhao, Julien Tailhades, and Max J. Cryle   10. Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe Akimasa Miyanaga, Fumitaka Kudo, and Tadashi Eguchi   11. A Practical Guideline to Engineering Non-Ribosomal Peptide Synthetases Nadya Abbood, Leonard Präve, Kenan A. J. Bozhueyuek, and Helge B. Bode   12. Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonance Neeru Arya, Kenneth A. Marincin, and Dominique P. Frueh   13. Ribosomal Synthesis of Peptides Bearing Noncanonical Backbone Structures via Chemical posttranslational modifications Yuki Goto and Hiroaki Suga   14. Thioester Capture Strategy for the Identification of Nonribosomal Peptide and Polyketide Intermediates Yueying Li, Lauren A. Washburn, and Coran M. H. Watanabe   15. Chemical Labeling of Protein 4′-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptide Synthetases Fumihiro Ishikawa and Genzoh Tanabe   Part III: Bioinformatics methods 16. Norine: Bioinformatics Methods and Tools for the Characterization of Newly Discovered NonRibosomal Peptides Areski Flissi, Matthieu Duban, Philippe Jacques, Valérie Leclère, and Maude Pupin

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