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OverviewThis volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors. Full Product DetailsAuthor: Nathaniel ShankPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1st ed. 2019 Volume: 1973 Weight: 0.843kg ISBN: 9781493992157ISBN 10: 1493992155 Pages: 315 Publication Date: 24 April 2019 Audience: College/higher education , Professional and scholarly , Postgraduate, Research & Scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs Stella Diafa, Damien Evéquoz, Christian J. Leumann, and Marcel Hollenstein 2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows 3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue Hui Mei and John Chaput 4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA Petra Ménová, Hana Cahová, Milan Vrábel, and Michal Hocek 5. 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari 6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids Tanaya Bose and Vaijayanti A. Kumar 7. Synthesis of Nucleobase-Functionalized Morpholino Monomers Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha 8. Synthesis and Application of LKγT Peptide Nucleic Acids Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella 9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya 10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates 11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids Malte Rosenthal, Franziska Pfeiffer, and Günter Mayer 12. Labeling Peptide Nucleic Acids with Indium-111 Igor G. Panyutin 13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg 14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates Maryline Chemama and Christopher Switzer 15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis Jung-Suk Choi and Anthony Berdis 16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA Renatus W. Sinkeldam and Yitzhak Tor 17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma 18. Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov 19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation Hailey L. Gahlon and Shana J. SturlaReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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