Overview

Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.

Full Product Details

Author:   M. A. Titheradge ,  Michael A Titheradge ,  M A Titheradge
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   New edition
Volume:   No. 100
Dimensions:   Width: 15.20cm , Height: 2.30cm , Length: 22.90cm
Weight:   0.765kg
ISBN:  

9780896035379

ISBN 10:   0896035379
Pages:   324
Publication Date:   23 December 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Book
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Enzymology of Nitric Oxide Synthases Benjamin Hemmens and Bernd Mayer. Purification of the Inducible Nitric Oxide Synthase Edward P. Garvey. Purification of the Constitutive Nitric Oxide Synthase Edward P. Garvey. Cloning and Expression of Human Inducible Nitric Oxide Synthase Mark L. Johnson, Richard A. Shapiro, and Timothy R. Billiar. Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System Ian G. Charles, Neale Foxwell, and Ann Chubb. Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO Mark Salter and Richard G. Knowles. Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation Richard G. Knowles and Mark Salter. Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation James M. Cunningham and Richard C. Rayne. The Enzymatic Measurement of Nitrate and Nitrite Michael A. Titheradge. Determination of NOS Activity Using Cyclic-GMP Formation Suzanne G. Laychock. Determination of NO with a Clark-Type Electrode Kurt Schmidt and Bernd Mayer. The Measurement of NO in Biological Systems Using Chemiluminescence Evangelos D. Michelakis and Stephen L. Archer. Measurement of NO Using Electron Paramagnetic Resonance S. Tsuyoshi Ohnishi. Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction Norbert Reiling, Artur J. Ulmer, and Sunna Hauschildt. Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay Paloma Martin-Sanz and Lisardo Bosca. Determination of NOS Isoforms by Western-Blot Analysis Fiona S. Smith and Michael A. Titheradge. Immunohistochemical Localization of NOS Isoforms Victoria Cattel and Karen Mosley. A Practical Protocol for the Demonstration of NOS Using In Situ Hybridization G. Michael Taylor and H. Terence Cook. Use of NO Donors in Biological Systems Jayne M. Tullettand Daryl D. Rees. Making and Working with Peroxynitrite C. Roger White, John P. Crow, Nathan Spear, Steven Thomas, Rakesh Patel, Irene Green, Joseph Beckman, and Victor Darley-Usmar. The Use of NO Gas in Biological Systems Neil Hogg and B. Kalyanaraman. A Microtiter-Plate Assay of Human NOS Isoforms John Dawson and Richard G. Knowles. Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings Rachel J. Russell. Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis Kzuyuki Hatakeyama. A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase IKazuyuki Hatakeyama and Toshie Yoneyama. Simultaneous Measurement of Mitochondrial Function and NO I gnacio Lizasoain and Maria A. Moro. Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen Kurt Schmidt, Wolfgang Desch, Peter Klatt, Walter R. Kukovetz, and Bernd Mayer. The Determination of Nitrotyrosine Residues in Proteins Andrew J. Gow, Molly McClelland, Sarah E. Garner, Stuart Malcom, and Harry Ischiropoulos. Measurement of DNA Damage Using the Comet Assay Steven Thomas, Michael H. L. Green, Jillian E. Lowe, and Irene C. Green. Methods for the Study of NO-Induced Apoptosis in Cultured Cells Anne C. Loweth and Noel G. Morgan. Index.

Reviews

the protocols are concise and easy to follow. They are, in fact, extraordinary in their detail Nitric Oxide Protocols is a useful volume, and one that will certainly find its way into many laboratories entering the exciting field of nitric oxide research. - The Quarterly Review of Biology

... the protocols are concise and easy to follow. They are, in fact, extraordinary in their detail...Nitric Oxide Protocols is a useful volume, and one that will certainly find its way into many laboratories entering the exciting field of nitric oxide research. -The Quarterly Review of Biology

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