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OverviewThis cutting-edge reference book compiles standard operating procedures, protocols, and applications of Next-Generation Sequencing (NGS). It discusses genomic testing applications through NGS. It pays special focus on the protocols for cataloging variants of uncertain significance. Over the years, NGS and advanced bioinformatics approaches have allowed the transition of genomic assays into translational practices. The book covers visualization of NGS data sets, investigation of early development impairment, and metagenome protocols. It also discusses the challenges in NGS methods. Key Points- · Includes case studies of application of next-generation sequencing in different taxa like humans, rodents, plants, bacteria · Compiles protocols from various reputed companies like Illumina, PacBio, ThermoFisher · Discusses the translational applications of NGS methods · Reviews Machine Learning Heuristics For NGS data interpretation · Discusses emerging genomic assay technologies and characterizing mechanisms of disease prevalence The book is meant for researchers and industry experts in genomics, computational biology, and bioinformatics. Full Product DetailsAuthor: Prashanth N Suravajhala , Jeff W BizzaroPublisher: Taylor & Francis Ltd Imprint: CRC Press ISBN: 9781032392622ISBN 10: 1032392622 Pages: 320 Publication Date: 19 December 2024 Audience: College/higher education , Professional and scholarly , Tertiary & Higher Education , Professional & Vocational Format: Hardback Publisher's Status: Forthcoming Availability: Not yet available  This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release. Table of Contents1. Technologies, Computations and Data Analysis for Next Generation Sequencing 2. Epigenetics: RNA-Seq, ChiP-Seq, MedIP-Seq and ATAC-Seq 3. Streamlining Next-Generation Sequencing Data Analysis with Nextflow and nf-core Pipelines 4. Best practices for variant calling using Genome Analysis Toolkit 5. Implementation of WGCNA for identifying regulatory modules in biological networks 6. Meta-analysis of RNA-seq and Microarray data 7. Best practices in single-cell RNA-seq data analysis 8. Integration of Spatial Transcriptomics and Single Cell RNA-Seq 9. Metagenomics Analysis Pipelines for Microbiome Studies: QIIME and Mothur 10. Standard Operating Procedure and Applications in Single-Cell Transcriptomics 11. Benchmarking and Evaluation of de novo Assembly Tools for Prokaryotic Long Reads from Oxford Nanopore Technologies 12. Best Practices for Reproducible of Microbial Genomics Analysis 13. Single Cell RNA-Seq Analyses in the Era of Artificial Intelligence 14. Towards single-molecule protein sequencing 15. SOPs on Effective Galaxy Workflows 16. Motif prediction using ChIP-Seq data analysis using Galaxy 17. Developing a Whole Exome Consensus Variant Calling Pipeline to Infer Causal Pathogenic Variants IndexReviewsAuthor InformationPrashanth N Suravajhala is a Principal Scientist, Systems Biology at the Amrita School of Biotechnology, Kerala, India. He has a PhD in Systems Biology from Aalborg University, Denmark. His group has benchmarked pipelines and developed methods for systems genomic integration. He is an Associate Editor of Frontiers in Genetics/Systems Biology, Bio-Protocol, Biomolecules, and BMC Medical Genomics. Jeff W Bizzaro has been involved in the field of bioinformatics for more than 25 years and has made significant contributions to the development of bioinformatics software. He is a co-founder of Bioinformatics.org, a professional network that advocates for open science and provides various resources to students and professionals. Tab Content 6Author Website:Countries AvailableAll regions |
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