Overview

This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.

Full Product Details

Author:   William S. Hancock (Northeastern University, Boston, Massachusetts, USA)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Volume:   2
Dimensions:   Width: 15.60cm , Height: 2.00cm , Length: 23.40cm
Weight:   0.570kg
ISBN:  

9780849378225

ISBN 10:   0849378222
Pages:   272
Publication Date:   23 October 1995
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Peptide Mapping to Characterize Subtle Modifications in the Analysis of Protein Pharmaceuticals, V.T. Ling, M.L. Eng, P.-J. Lee, R.G. Keck, B.A. Keyt, and E.Canova-Davis The Optimization of Peptide Mapping via Computer Simulation, L.R. Snyder The Use of Capillary Electrophoresis for Peptide Mapping of Proteins, E. Rickard and J. Towns Packed Capillary HPLC-ELectrospray Ionization Mass Spectrometry, J. Stults, B.L. Gillece-Castro, W.J. Henzel, J.H. Bourell, K.L. O'Connell, and L.M. Nuwaysir Capillary Electrophoresis/Mass Spectrometry in Peptide Mapping, J. Wahl, H. Udseth, and R. Smith Analyzing Reversed Phase Peptide Maps of Recombinant Glycoproteins, A. Guzzetta, W. Hancock Matrix-Assisted Laser Desorption Mass Spectrometry for Peptide Mapping, P. Lecchi and R. Caprioli

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