Overview

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Full Product Details

Author:   Dobrin Nedelkov ,  Randall W. Nelson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2006 ed.
Volume:   328
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.587kg
ISBN:  

9781588295194

ISBN 10:   1588295192
Pages:   248
Publication Date:   01 March 2006
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

On-Chip Protein Synthesis for Making Microarrays.- RCA-Enhanced Protein Detection Arrays.- Antibody Microarrays Using Resonance Light-Scattering Particles for Detection.- Chemical Proteomics Profiling of Proteasome Activity.- Two-Dimensional Difference Gel Electrophoresis.- Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors.- Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays.- Surface Plasmon Resonance Mass Spectrometry for Protein Analysis.- High-Throughput Affinity Mass Spectrometry.- Isotope-Coded Affinity Tags for Protein Quantification.- Proteomic Analysis by Multidimensional Protein Identification Technology.- Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites.- N-Glycosylation Analysis Using the StrOligo Algorithm.- MALDI-MS Data Analysis for Disease Biomarker Discovery.- Using the Global Proteome Machine for Protein Identification.

Reviews

From the reviews: It is no surprise that a book of this title is somewhat eclectic. ! Methodologies are well written and useful, emphasizing that proteins and function can be studied at differing levels of resolution. ! this book will be useful for beginners, though it is really more for the initiated seeking inspiration. (Dr. Mike J. Naldrett, Microbiology Today, November, 2006)

From the reviews: <p> It is no surprise that a book of this title is somewhat eclectic. a ] Methodologies are well written and useful, emphasizing that proteins and function can be studied at differing levels of resolution. a ] this book will be useful for beginners, though it is really more for the initiated seeking inspiration. (Dr. Mike J. Naldrett, Microbiology Today, November, 2006)

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