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OverviewNeuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. The target audience includes clinical or basic scientists who look to apply proteomics to the neurosciences. Often researchers hear of proteomics without an adequate explanation of the methodology and inherent limitations. This volume conveys where proteomic methodology is in its application to CNS research and what results can be expected. We also address clinical translation of neuroproteomics, specifically in the area of biomarker research. The inception of neuroproteomics capitalized on rapid progress in large-molecule mass spectrometry over the last decade. Two seminal advances have spurred research – development of reliable polypeptide ionization processes and bioinformatics to rapidly process tandem mass spectra for peptide identification and quantification. What has followed is the exponential application of mass spectrometry to proteome characteri- tion across biological and biomedical disciplines. Arguably, the most elaborate proteomic implementation is in studying the CNS, the most enigmatic and complex animal system. Neuroscience is characterized by grandiose questions – what is consciousness, how does thought or memory work. Neuroproteomics researchers, however, have pri- rily involved themselves dysfunction, based on a pressing need (and invariably funding), in answering questions on CNS dysfunction, based on a pressing need (and invariably funding), and because such questions hold more accessible answers. Dysfunction is readily contrasted against normal function and presumably produces a lasting differential protein signature. Full Product DetailsAuthor: Andrew K. Ottens , Kevin K.W. WangPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2009 Volume: 566 Weight: 0.921kg ISBN: 9781934115848ISBN 10: 1934115843 Pages: 321 Publication Date: 05 August 2009 Audience: College/higher education , Postgraduate, Research & Scholarly Format: Hardback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsDisease Models in Neuroproteomics.- The Methodology of Neuroproteomics.- Modeling Cerebral Ischemia in Neuroproteomics.- Clinical and Model Research of Neurotrauma.- Neuroproteomic Methods in Spinal Cord Injury.- Modeling Substance Abuse for Applications in Proteomics.- Protein Aggregate Characterization in Models of Neurodegenerative Disease.- Sub-Proteome Separations and Neuroproteomic Analysis.- Sub-Proteome Processing: Isolation of Neuromelanin Granules from the Human Bra.- Proteomic Analysis of Protein Phosphorylation and Ubiquitination in Alzheimer’s Disease.- Proteomics Identification of Carbonylated and HNE-Bound Brain Proteins in Alzheimer’s Disease.- Mass Spectrometric Identification of In Vivo Nitrotyrosine Sites in the Human Pituitary Tumor Proteome.- Improved Enrichment and Proteomic Analysis of Brain Proteins with Signaling Function by Heparin Chromatography.- Calmodulin-Binding Proteome in the Bra.- Neuroproteomic Methodology and Bioinformatics.- Separation of the Neuroproteome by Ion Exchange Chromatography.- iTRAQ-Based Shotgun Neuroproteomics.- Methods in Drug Abuse Neuroproteomics: Methamphetamine Psychoproteome.- Shotgun Protein Identification and Quantification by Mass Spectrometry in Neuroproteomics.- Biofluid Analysis and Clinical Translation.- Identification of Glycoproteins in Human Cerebrospinal Fluid.- Mass Spectrometric Analysis of Body Fluids for Biomarker Discovery.- Traumatic Brain Injury Biomarkers: From Pipeline to Diagnostic Assay Development.- Translation of Neurological Biomarkers to Clinically Relevant Platforms.ReviewsFrom the reviews: This book provides experimental details about animal models for neuroproteomic research, methods for separating and analyzing discrete subcomponents of the neuroproteome, and CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. ... The authors intend the book for basic and clinical researchers, and it will be of much interest to basic scientists, clinical scientists, and students interested in applying proteomics to the neurosciences. (Omer Iqbal, Doody's Review Service, March, 2010) From the reviews: This book provides experimental details about animal models for neuroproteomic research, methods for separating and analyzing discrete subcomponents of the neuroproteome, and CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. ... The authors intend the book for basic and clinical researchers, and it will be of much interest to basic scientists, clinical scientists, and students interested in applying proteomics to the neurosciences. (Omer Iqbal, Doody's Review Service, March, 2010) From the reviews: This book provides experimental details about animal models for neuroproteomic research, methods for separating and analyzing discrete subcomponents of the neuroproteome, and CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. ! The authors intend the book for basic and clinical researchers, and it will be of much interest to basic scientists, clinical scientists, and students interested in applying proteomics to the neurosciences. (Omer Iqbal, Doody's Review Service, March, 2010) From the reviews: “This book provides experimental details about animal models for neuroproteomic research, methods for separating and analyzing discrete subcomponents of the neuroproteome, and CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. … The authors intend the book for basic and clinical researchers, and it will be of much interest to basic scientists, clinical scientists, and students interested in applying proteomics to the neurosciences.” (Omer Iqbal, Doody’s Review Service, March, 2010) Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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