Overview

This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Marcel M. Daadi
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1919
Weight:   0.897kg
ISBN:  

9781493990054

ISBN 10:   1493990055
Pages:   239
Publication Date:   18 January 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Generation of Neural Stem Cells from Induced Pluripotent Stem Cells Marcel M. Daadi 2. Making NSC and Neurons from Patient Derived Tissue Samples Odity Mukherjee, Shubhra Acharya, and Mahendra Rao 3. Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury Mohamad Khazaei , Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, and Michael G. Fehlings 4. Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells Rodolfo Gonzalez, Ibon Garitaonandia, and Russell Kern 5. Chemically Defined Neural Conversion of Human Pluripotent Stem Cells Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov, and Ilyas Singec 6. In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology Marita Gronning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, and Zaal Kokaia 7. Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons Marcel M. Daadi 8. Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells Fabiano Tofoli de Araujo, Ana Teresa Silva Semeano, Agatha Oliveira-Giacomelli, Maria Carolina Bittencourt Goncalves, Merari de Fatima Ramires Ferrari, Lygia da Veiga Pereira,and Henning Ulrich 9. Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene Hyenjong Hong and Marcel M. Daadi 10. Single-Cell Library Preparation of iPSC-derived Neural Stem Cells Jeffrey Kim and Marcel M. Daadi 11. Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells Jeffrey Kim and Marcel M. Daadi 12. Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons. Gourav R. Choudhury and Marcel M. Daadi 13. Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi 14. Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, and Peter J. Hornsby 15. Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells Hyenjong Hong, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi 16. Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation Cang Chen, Michael Guderyon, Guo Ge, Robert A Clark, and Senlin Li 17. Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert, Yuji Kaneko and Cesar V. Borlongan 18. Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion Shijie Song, Xiaoyung Kong, and Juan Sanchez-Ramos

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