Overview

This is a collection of cutting-edge mycoplasma methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenecity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products.

Full Product Details

Author:   Roger J. Miles ,  Robin A. J. Nicholas
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   104
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.758kg
ISBN:  

9780896035256

ISBN 10:   0896035255
Pages:   330
Publication Date:   25 May 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Medical Significance of Mycoplasmas.- The Veterinary Significance of Mycoplasmas.- Recovery of Human Mycoplasmas.- Recovery of Mycoplasmas from Animals.- Recovery of Mycoplasmas from Birds.- Cultivation of Ureaplasmas.- Quality Control Testing of Mycoplasma Medium.- Biochemical Characteristics in Mycoplasma Identification.- Enzyme Analysis.- Determination of Substrate Utilization Rates by Mycoplasmas.- Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests.- Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot).- Identification of Mycoplasmas by Immunofluorescence.- Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs.- Immunohistochemical Staining of Fixed Tissues.- Extraction of DNA from Mycoplasmas.- Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers.- PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC.- Characterization of Mycoplasmas by RAPD Fingerprinting.- Classification of Isolates by DNA-DNA Hybridization.- Insertion Sequence Analysis.- Detection of Mycoplasmas in Cell Cultures by Cultural Methods.- Detection of Mycoplasmas in Cell Culture by Fluorescence Methods.- Transformation of Mycoplasmas.- Transposon Mutagenesis.- Demonstration of Extrachromosomal Elements.- Expression of Foreign Genes in Acholeplasma laidlawii.- Mycoplasma Gene Expression in Escherichia coli.- Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins.- Immunological and Biochemical Characterization of Membrane Proteins.- Detection and Analysis of Mycoplasma Adhesins.- Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens.

Reviews

The value of this book lies in the amount of technical detail provided...this should enable the interested investigator to carry out the protocols described without recourse to other publications. - Journal of Medical Microbiology

The value of this book lies in the amount of technical detail provided. . .this should enable the interested investigator to carry out the protocols described without recourse to other publications. -Journal of Medical Microbiology

The value of this book lies in the amount of technical detail provided...this should enable the interested investigator to carry out the protocols described without recourse to other publications. -Journal of Medical Microbiology

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