Overview

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

Full Product Details

Author:   T. Parish ,  N.G. Stoker
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   v. 101
Dimensions:   Width: 23.40cm , Height: 2.60cm , Length: 15.60cm
Weight:   0.862kg
ISBN:  

9780896034716

ISBN 10:   0896034712
Pages:   492
Publication Date:   08 February 2010
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Mycobacteria: Bugs and Bugbears Tanya Parish and Neil G. Stoker. Mycobacteria: General Culture Methodology and Safety Considerations Bryan W. Allen. Isolation of Genomic DNA from Mycobacteria John T. Belisle and Michael G. Sonnenberg. Nucleic-Acid Extraction: Plasmid/Cosmid DNA Bonnie B. Plikaytis and C. Harold King. Pulsed Field Gel Electrophoresis for Mycobacteria Wolfgang J. PhilippStephen Gordon Amalio Telenti and Stewart T. Cole. Extraction of RNA from Mycobacteria Eshwar Mahenthiralingam. Preparation of Cell-Free Extracts from Mycobacteria Tanya Parish and Paul R. Wheeler. Preparation of Cell-Wall Fractions from Mycobacteria Gurdyal S. Besra. Using MycDB on the World-Wide Web Staffan Bergh and Stewart T. Cole. Conjugating DNA into Mycobacteria Julie-Ann Gavigan and Carlos Martin. Electroporation of Mycobacteria Tanya Parish and Neil G. Stoker. Mycobacteriophages Gary J. Sarkis and Graham F. Hatfull. Chemical Mutagenesis of MycobacteriaLucy A. Brooks. Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System Esther P'rez Julie-Ann Gavigan Isabel Otal Christophe Guilhot Vladimir Pelicic Brigitte Giguel and Carlos Martin. Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement Robert N. Husson. Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker Peter Sander and Erik C. Bttger. Gene Replacement in Mycobacterium intracellulare Britt-Inger Marklund and Richard W. Stokes. Gene Replacement in Mycobacterium bovis BCG Elizabeth Norman. The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria Stephen Gordon Gavin Chung and Peter Andrew. Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria Laura E. Via Subramanian Dhandayuthapani Dusanka Deretic and V. Deretic. Expression of Genes in Mycobacteria Peadar - Gaora. Secretion of Mammalian Proteins from Mycobacteria Peter J. Murray and Richard A. Young. Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR Philip D. Butcher Joseph A. Mangan and Irene M. Monahan.Analysis of Mycobacterial Differential Gene Expression by RAP-PCR Joseph A. Mangan and Philip D. Butcher. Mycobacterial Speciation Francis A. Drobniewski and Anne H. C. Uttley. Species Identification of Mycobacteria Using rDNA Sequencing Philip Kirschner and Erik C. Bttger. Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction Stuart M. Wilson. Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping Henri O. F. Molhuizen Annelies E. Bunschoten Leo M. Schouls and Jan D. A. van Embden. Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis Herre F. Heersma Kristin Kremer and Jan D. A. van Embden. Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism) Amalio Telenti Nadine Honor' and Stewart T. Cole. The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria Paul F. Riska and William R. Jacobs Jr. Index.

Reviews

this book is an essential purchase for an laboratory dealing with mycobacteria and good value for individual researchers. - Microbiology Today a unique book presents excellent description of many of the molecular biological tools that have been developed an invaluable collection of methodologies for mycobacterial research the molecular mycobacteriologist will find much of interest in this book Worth getting. - Journal of Medical Microbiology ...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms...of interest to the epidemiologist is the description of computer analysis of DNA fingerprinting for tuberculosis while the chapter on accessing MycDB, a database dedicated to mycobacterial research, will be of value to all those working in this area. - Molecular Biotechnology this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms. The detailed technical nature of each chapter provides clear instruction for those wishing to engage in this area of research. - Molecular Biotechnology

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