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OverviewMutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field. Full Product DetailsAuthor: R. G. H. Cotton (Mutation Research Centre, Melbourne) , E. Edkins (Joint Women's and Children's Hospital, Perth) , S. Forrest (Murdoch Institute, Parkville) , S. Forrest (Murdoch Institute, Parkville, Australia)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 188 Dimensions: Width: 15.70cm , Height: 1.50cm , Length: 23.50cm Weight: 0.382kg ISBN: 9780199636563ISBN 10: 0199636567 Pages: 262 Publication Date: 05 February 1998 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of Contents1: Single strand conformation polymorphism analysis 2: Single-stranded conformation polymorphism and heteroduplex analysis 3: Comprehensive mutation detection with denaturing gradient gel electrophoresis 4: Cleavage using RNase to detect mutations 5: Cleavage of mismatched bases using chemical reagents 6: Mutation detection using T4 endonuclease VII 7: Detection of mutations by hybridization with sequence-specific oligonucleotide probes 8: DNA detection and sequence distinction through oligonucleotide ligation 9: Detection of sequence variation using primer extension 10: Selective amplification of specific alleles 11: The protein truncation text (PTT) 12: Functional assay of the p53 tumour suppressor gene 13: Advances in direct DNA sequencing for mutation scanningReviewsThis book will be useful to laboratory workers in both biolgoy and medicine. It provides practical guidance on tested protocols covering a range of detection methods, including both scanning mode, used for unknown mutations, and the diagnostic mode which tests for know mutations. This practical manual is a welcomed addition to this well-known series. ASLIB Book Guide, Vol.63, no.7 '...will provide a valuable resource both for those new to the area and for more experienced mutation detectives...' Trends in Genetics vol.15, no.1 `This book will be useful to laboratory workers in both biolgoy and medicine. It provides practical guidance on tested protocols covering a range of detection methods, including both scanning mode, used for unknown mutations, and the diagnostic mode which tests for know mutations. This practical manual is a welcomed addition to this well-known series.' ASLIB Book Guide, Vol.63, no.7 `'...will provide a valuable resource both for those new to the area and for more experienced mutation detectives...'' Trends in Genetics vol.15, no.1 '...will provide a valuable resource both for those new to the area and for more experienced mutation detectives...' * Trends in Genetics vol.15, no.1 * This book will be useful to laboratory workers in both biolgoy and medicine. It provides practical guidance on tested protocols covering a range of detection methods, including both scanning mode, used for unknown mutations, and the diagnostic mode which tests for know mutations. This practical manual is a welcomed addition to this well-known series. * ASLIB Book Guide, Vol.63, no.7 * Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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