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OverviewThis detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vitalresource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes. Full Product DetailsAuthor: Claudia BindaPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2023 Volume: 2558 Weight: 0.700kg ISBN: 9781071626429ISBN 10: 1071626426 Pages: 254 Publication Date: 29 September 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Purification of MAO A and of MAO B from Mammalian Tissue Sources Dale E. Edmondson 2. Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System Dale E. Edmondson 3. The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity Joana Reis and Claudia Binda 4. Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays Joana Reis and Claudia Binda 5. Radiochemical Assay of Monoamine Oxidase Activity Andrew Holt 6. MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition Rona R. Ramsay 7. Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase Andrew Holt 8. Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS) Tomás Herraiz 9. Crystallization of Human Monoamine Oxidase B Claudia Binda, Dale E. Edmondson, and Andrea Mattevi 10. Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, and Darrell D. Mousseau 11. An (Immune)Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution within the Cell Tyler J. Wenzel, Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Chris P. Phenix, and Darrell D. Mousseau 12. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene-Transfer Yohan Santin, Angelo Parini, and Jeanne Mialet-Perez 13. In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers Boyang Jason Wu and Jean C. Shih 14. In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis Philippe De Deurwaerdère, Nouhad Samb, Hasna El Boukhari, Rémi Corne, Abdeslam Chagraoui, and Giuseppe Di Giovanni 15. Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors Nicola Gambacorta, Marco Catto, Leonardo Pisani, Angelo Carotti, and Orazio Nicolotti 16. Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology Leonardo Pisani, Modesto de Candia, Mariagrazia Rullo, and Cosimo D. Altomare 17. Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors Kemal Yelekçi and Safiye Sağ ErdemReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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