Overview

This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an “integrative taxonomy” framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date,Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.

Full Product Details

Author:   Pascale Besse
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2222
Weight:   0.976kg
ISBN:  

9781071609965

ISBN 10:   1071609963
Pages:   400
Publication Date:   11 December 2020
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives             Germinal Rouhan and Myriam Gaudeul   2. Guidelines for the Choice of Sequences for Molecular Plant Taxonomy             Pascale Besse   3. Isolation and Purification of DNA from Complicated Biological Samples             Ruslan Kalendar, Svetlana Boronnikova, and Mervi Seppänen   4. Herbarium Specimens: A Treasure for DNA Extraction, an Update             Lenka Záveská Drábková   5. Sequencing of Complete Chloroplast Genomes             Berthold Heinze   6. Utility of the Mitochondrial Genome in Plant Taxonomic Studies             Jérôme Duminil and Guillaume Besnard   7. Nuclear Ribosomal RNA Genes: ITS Region             Pascale Besse   8. Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla             Pascale Besse, Denis Da Silva, and Michel Grisoni   9. High-Throughput Genotyping Technologies in Plant Taxonomy             Monica F. Danilevicz, Cassandria T. Fernandez, Jacob I. Marsh, Philipp E. Bayer, and David Edwards   10. Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny             Félicien Favre, Cyril Jourda, Pascale Besse, and Carine Charron   11. Development of Microsatellite Markers Using Next-Generation Sequencing             Hélène Vignes and Ronan Rivallan   12. Amplified Fragment Length Polymorphism: Applications and Recent Developments             Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, and Kantipudi Nirmal Babu   13. Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques             Kantipudi Nirmal Babu, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby, and Illathidath Payatatti Vijesh Kumar   14. Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers             Chrissen E.C. Gemmill and Ella R.P. Grierson   15. Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity             Ruslan Kalendar, Alexander Muterko, and Svetlana Boronnikova   16. Introduction to Population Genomics Methods             Thibault Leroy and Quentin Rougemont   17. The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants             Jaume Pellicer, Robin F. Powell, and Ilia J. Leitch   18. Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization             Sonja Siljak-Yakovlev, Fatima Pustahija, Vedrana Vicic Bockor, and Odile Robin   19. GISH: Resolving Interspecific and Intergeneric Hybrids             Nathalie Piperidis

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