Overview

Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-protein interaction methods are also covered in detail. Inducible gene expression in plants as well as expression and analysis of recombinant proteins, and analysis of protein import into chloroplasts are covered as well as techniques for fractionation of plant tissue for biochemical analyses and the study of protein-protein interactions with the yeast two-hybrid system. A range of approaches for using antibodies as tools are also described including the use of antibody phage display libraries. The final section on emerging technologies describes methodologies for calcium imaging and for the spatial and temporal analysis of reporter genes such as luciferase and green fluorescent protein. The final area covers a range of experimental procedures for moss, which is emerging as a new model organism.

Full Product Details

Author:   Philip M Gilmartin (Director, Centre for Plant Sciences, University of Leeds) ,  Chris Bowler (Head, Laboratory of Molecular Plant Biology, Stazione Zoologica, Napoli, Italy)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   259
Dimensions:   Width: 19.00cm , Height: 2.00cm , Length: 24.70cm
Weight:   0.816kg
ISBN:  

9780199638185

ISBN 10:   0199638187
Pages:   358
Publication Date:   20 June 2002
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Part 1: Gene Expression 1: Transcript analysis 2: In situ hybridization 3: DNA microarrays for gene expression analysis 4: DNA-protein interactions 5: Inducible gene expression in plants Part 2: Gene Product Analysis 6: Expression of recombinant proteins 7: Import of proteins into isolated chloroplasts and thylakoid membranes 8: Fractionation of plant tissue for biochemical analysis 9: Studying protein-protein interactions with the yeast two-hybrid system 10: Antibody techniques 11: Antibody phage display libraries Part 3: Emerging Technologies 12: Spatial and temporal measurements of calcium ions in living plant cells 13: Reporter genes for plant cells 14: Moss gene technology

Reviews

The chapters are well written, with sufficient background detail and a step-by-step guide to each technique. Overall, this will be a useful manual for any plant molecular biology laboratory. Microbiology Today

The chapters are well written, with sufficient background detail and a step-by-step guide to each technique. Overall, this will be a useful manual for any plant molecular biology laboratory. Microbiology Today

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