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OverviewMatthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of essential, state-of-the-art techniques in developmental studies, and offers experienced researchers time-saving tips and new techniques from experts who have fine-tuned them for best results. Full Product DetailsAuthor: Matt GuillePublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 1st ed. 1999 Volume: 127 Dimensions: Width: 15.20cm , Height: 1.20cm , Length: 22.90cm Weight: 0.401kg ISBN: 9781617371899ISBN 10: 1617371890 Pages: 217 Publication Date: 10 November 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsThe Animal Cap Assay.- Cell and Tissue Transplantation in Zebrafish Embryos.- Ribonuclease Protection Analysis of Gene Expression in Xenopus.- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos.- In Situ Hybridization to Sections of Xenopus Embryos.- Zebrafish Immunohistochemistry.- Immunohistochemistry of Xenopus Embryos.- Preparation and Testing of Synthetic mRNA for Microinjection.- Microinjection into Xenopus Oocytes and Embryos.- Microinjection into Zebrafish Embryos.- Expression from DNA Injected into Xenopus Embryos.- Promoter Analysis in Zebrafish Embryos.- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs.- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis.- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis.- Mapping Protein-DNA Interactions Using In Vivo Footprinting.ReviewsReaders will find the systematic description of methods, illustration of structures, tables and figures, along with comments on optimizing the methods for use in other systems, very useful. ...this excellent book will facilitate studies on the lipidation of proteins. It should be useful to both beginning and experienced investigators in the field. - Analytical Biochemistry ...this compact book comprises concise and very well structured chapters on both basic and advanced molecular and embryological technique used in studies of early stages of vertebrate development on two model species, the South Africa clawed frog Xenopus loevis and the zebrafish Brachydanio rerio....All chapters have been written by authors with first-hand expertise in individual fields, mostly those in whose laboratories the methods in question have been developed, or at least optimized. Close attention is paid to meticulous description of possible pitfalls and drawbacks, and essential steps are stressed....the book contains a wealth of information, cues on methodology and their critical assessment. It is an almost indispensable guide for anybody working in the field of vertebrate development biology. -Folia Microbiologica ...this book is a solid introduction to some of the common techniques in zebrafish and Xenopus and I think would be of good use to its intended audience of new workers in the field. - Newsletter of the British Society of Developmental Biology Readers will find the systematic description of methods, illustration of structures, tables and figures, along with comments on optimizing the methods for use in other systems, very useful. ...this excellent book will facilitate studies on the lipidation of proteins. It should be useful to both beginning and experienced investigators in the field. - Analytical Biochemistry ...this compact book comprises concise and very well structured chapters on both basic and advanced molecular and embryological technique used in studies of early stages of vertebrate development on two model species, the South Africa clawed frog Xenopus loevis and the zebrafish Brachydanio rerio...All chapters have been written by authors with first-hand expertise in individual fields, mostly those in whose laboratories the methods in question have been developed, or at least optimized. Close attention is paid to meticulous description of possible pitfalls and drawbacks, and essential steps are stressed...the book contains a wealth of information, cues on methodology and their critical assessment. It is an almost indispensable guide for anybody working in the field of vertebrate development biology. -Folia Microbiologica ...this book is a solid introduction to some of the common techniques in zebrafish and Xenopus and I think would be of good use to its intended audience of new workers in the field. - Newsletter of the British Society of Developmental Biology Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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