Overview

This book describes and discusses experimental procedures used in a wide range of specialized topics within the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Contributions are by internationally-recognized experts in particular areas and represent up-to-date methods currently in use. Topics covered include DNA isolation, cloning, and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolation, Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast. Detailed protocols are clearly presented, with many additional practical tips, to assist both first-time and more experienced experimentalists.

Full Product Details

Author:   John R. Johnston (Reader in Microbial Genetics, University of Strathclyde)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   141
Dimensions:   Width: 14.50cm , Height: 1.70cm , Length: 23.40cm
Weight:   0.432kg
ISBN:  

9780199634293

ISBN 10:   0199634297
Pages:   300
Publication Date:   30 June 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1: Procedures for isolating yeast DNA for different purposes 2: Construction of cloning and expression vectors 3: Cosmid cloning of yeast DNA 4: The construction and use of cDNA libraries for genetic selections 5: Pulsed field gel electrophoresis 6: Plasmid shuffling and mutant isolation 7: Ty insertional mutagenesis 8: High efficiency transformation with lithium acetate 9: Measurement of transcription 10: Measurement of mRNA stability 11: Production of foreign proteins at high level 12: Cell-free translation of natural and synthetic mRNAs 13: Virus-like particles: Ty retrotransposons 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly 15: Immuno-electron microscopy 16: Industrial Saccharomyces yeasts

Reviews

The distinguished collection of authors are often pioneers in their subject area and so the guidance is both authoritative and helpful. Overall this is a useful addition to the series, with ready access to standard lab protocols. Alan Wheals, Society for General Microbiology, November 1996

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