Overview

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

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9780879695774

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Any basic research laboratory using molecular biology techniques will benefit from having a copy on hand of the newly published Third Edition of Molecular Cloning: A Laboratory Manual, by Joe Sambrook and David Russell... Originally published almost 20 years ago, the first two editions of this book have been staples of molecular biology with a proven reputation for accuracy and thoroughness. The Scientist Molecular Cloning: A Laboratory Manual has always been the laboratory mainstay for protocols and techniques. It has a pure-bred ancestry, and the new addition does not disappoint. Sambrook and Russell have built upon the basic principles that made the first two editions essential reference resources. Protocols that cover everything possible in a molecular biology lab are explained in detail. ...The new edition includes information panels at the end of each chapter that describe the principles behind the protocols... The addition of this information extends Molecular Cloning from an essential laboratory resource into a new realm, one merging the previous prototype with a modern molecular monograph... It is difficult to find fault with the first two editions of Molecular Cloning. For decades, their superior presentation of laboratory protocols made them the gold standard in the field of molecular biology. However, in many laboratories the old editions are becoming worn out-a testament to their continual use. For most, it is time to retire these workhorses and allow them some peace. They and you will be safe in the knowledge that the next generation of Molecular Cloning not only carries on the proud heritage of the first two editions but also admirably expands on that tradition to provide a truly essential laboratory manual. Trends in Microbiology In every kitchen there is at least one indispensable cookbook. Sambrook and Russell's Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory. Like its kitchen counterparts (e.g. Rombeck's Joy of Cooking) Sambrook's Molecular Cloning (MC) has information to help both the inexperienced and the advanced user. The analogy is apt, as MC is set up, and reads, very much like a molecular cookbook. Like a good cookbook, it has good logistics. Moreover, like 'Joy', the authors provide a wealth of theoretical (and historical) background for each experimental paradigm. MC is well organized, with a thorough index and detailed table of contents... MC provides the user with detailed information about the characteristics of reagents, supplies and kits from various manufacturers-all of which is important in our age of 'kit science'. The authors point out the pros and cons involved in using such reagents and kits, and recommend them at times (e.g. for cDNA synthesis and library construction), but stress caution in others (e.g. switching to non-radioactive detection methods). We applaud the inclusion of protocols even when kits are suggested, so users can be aware of the traditional methods that accomplish the same tasks... with this new edition and its companion website, MC has once again established its primacy as the molecular laboratory manual and is likely to be found on lab benches, and book-marked on web browsers, around the world. Trends in Neurosciences

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