Overview

Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations.

Full Product Details

Author:   Jacqueline Boultwood ,  Carrie Fidler
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2002 ed.
Volume:   68
Dimensions:   Width: 15.20cm , Height: 2.50cm , Length: 22.90cm
Weight:   0.668kg
ISBN:  

9780896036222

ISBN 10:   0896036227
Pages:   304
Publication Date:   10 October 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Molecular Analysis of Cancer.- Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization.- Spectral Karyotyping in Cancer Cytogenetics.- Comparative Genomic Hybridization Analysis.- Detection of Chromosomal Deletions by Microsatellite Analysis.- Detection and Quantification of Leukemia-Specific Rearrangements.- Detection of t(2;5)(p23;q35)Translocation by Long-Range PCR of Genomic DNA.- Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer.- Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism.- Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis.- Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay.- Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay.- Mutation Analysis of Cancer Using Automated Sequencing.- Detection of Differentially Expressed Genes in Cancer Using Differential Display.- Genomewide Gene Expression Analysis Using cDNA Microarrays.- Gene Expression Profiling in Cancer Using cDNA Microarrays.- Wilms Tumor Gene WT1 as a.- Detection of Aberrant Methylation of the p15 INK4B Gene Promoter.- Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon.- 20Telomere Length Changes in Human Cancer.- Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders.

Reviews

The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher. -Doody's Health Sciences Book Review Journal This book gives a good overview of currently available methods for investigating molecular changes in cancers,...it illustrates how you, the cancer researcher, can apply the methods to your oncology problem...The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping...a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available. - Molecular Biotechnology Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities. - Clinical Laboratory ...good integration between preliminary explanations of techniques and practical instructions with easily available tables or figures. - Proteomics

The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher. - Doody's Health Sciences Book Review Journal This book gives a good overview of currently available methods for investigating molecular changes in cancers, it illustrates how you, the cancer researcher, can apply the methods to your oncology problem The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available. - Molecular Biotechnology Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities. Clinical Laboratory

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